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Selective Assay of Monomeric and Filamentous Actin in Cell Extracts, Using Inhibition of Deoxyribonuclease I

Overview
Journal Cell
Publisher Cell Press
Specialty Cell Biology
Date 1978 Nov 1
PMID 728995
Citations 120
Authors
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Abstract

A simple and selective assay for monomeric and filamentous actin is presented, based on the inhibition of DNAase I by actin. In mixtures of monomeric and filamentous actin, only the monomeric form is measured as DNAase inhibitor. The total amount of actin in a sample can be determined after depolymerization of F actin with guanidine hydrochloride. The assay is rapid enough to detect changes in the polymerization state of actin in vitro over time intervals as short as 3 min. Data characterizing unpolymerized and filamentous actin pools in extracts of human platelets, lymphocytes and HeLa cells are presented.

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