Induction of Human Antigen-specific Suppressor Factors in Vitro

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1981 Mar 1

PMID 6169475

Citations 9

Authors

S Kontiainen

J N Woody

A Rees

M Feldmann

Affiliations

Soon will be listed here.

Abstract

Based on methods used for the in vitro induction of antigen-specific suppressor cells in the mouse, we have cultured Ficoll-Isopaque-separated human blood cells with high dose of antigen (100 microgram/ml) in Marbrook culture vessels for 4 days. The resulting cells, when further recultured for 24 hr with a low dose of antigen (1 microgram/ml), released into the supernatant material, termed 'suppressor factor', which inhibited, in an antigen-specific manner, the antibody response of mouse spleen cells in culture. The suppressor factor was analysed using immunoabsorbents, and was bound to and eluted from specific antigen, concanavalin A and lentil lectin, anti-human Ia antibodies, and anti-mouse suppressor factor antibodies, but was not bound to antibodies against human IgG.

Citing Articles

A comparative study of streptococcal antigen-binding human T8+ cells and monocytes, in relation to the HLA-DRw6 locus and the helper, suppressor and contrasuppressor functions.

Lehner T, Jones T Immunology. 1984; 53(2):215-25.

PMID: 6237980 PMC: 1454812.

Human insulin specific suppressor factors. I. Induction, characteristics and comparison to murine factors.

Kontiainen S Clin Exp Immunol. 1982; 47(3):715-24.

PMID: 6211311 PMC: 1536437.

Cell-bound helper and suppressor factors in primate lymphocytes.

Lehner T, Lamb J, Kontiainen S Clin Exp Immunol. 1982; 47(3):706-14.

PMID: 6211310 PMC: 1536432.

Structure of antigen-specific suppressor factors: current views.

Kontiainen S, James R, Culbert E, Feldmann M Surv Immunol Res. 1982; 1(1):17-23.

PMID: 6195709 DOI: 10.1007/BF02918237.

Characteristics of suppressor factors generated from human mononuclear cells using concanavalin A and C-reactive protein.

WANGEL A, Kontiainen S Clin Exp Immunol. 1983; 53(2):451-7.

PMID: 6192956 PMC: 1535698.

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