A Monoclonal Antibody That Detects a V Kappa-TEPC15 Idiotypic Determinant Cross-reactive with a Thy-1 Determinant

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1981 May 1

PMID 6166711

Citations 20

Authors

E Pillemer

I L Weissman

Affiliations

Soon will be listed here.

Abstract

To identify T lymphocyte antigens with immunoglobulin-like determinants, we prepared rat anti-mouse T cell monoclonal antibodies and screened them against a panel of purified mouse myeloma proteins representing all isotypes of immunoglobulin. One hybridoma, designated 42-21, was found to detect a novel antigenic determinant shared by V kappa-TEPC15 and the Thy-1 molecule on all T lymphocytes. Although several explanations for this unusual phenomenon exist, it may imply some role for the Thy-1 molecule in antigen and/or mitogen recognition. In any event, future studies of idiotypes on T lymphocytes must consider the possibility that anti-idiotypic sera detect cell surface molecules unrelated to classical immunoglobulin.

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