Mouse Beta 2-microglobulin CDNA Clones: a Screening Procedure for CDNA Clones Corresponding to Rare MRNAs

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1981 Apr 1

PMID 6166005

Citations 146

Authors

J R Parnes

B Velan

A Felsenfeld

L Ramanathan

U FERRINI

E Appella

J G Seidman

Affiliations

Soon will be listed here.

Abstract

We have isolated three cDNA clones for beta 2-microglobulin, the small subunit of the major histocompatibility antigens. beta 2-Microglobulin makes up less than 0.1% of mouse liver protein, and its mRNA is approximately 0.03% of liver poly(A)+ mRNA. The cDNA clones were identified by screening 1400 cDNA clones made from 9--10S mouse liver poly(A)+ mRNA. The procedure for screening the cDNA clones involved binding pooled plasmid DNA to nitrocellulose filters and testing the ability of each filter to select beta 2-microglobulin mRNA. The filter-selected mRNAs were assayed for their ability to direct the synthesis of beta 2-microglobulin in translation reactions in vitro. The isolated clones were shown by nucleotide sequence analysis to encode beta 2-microglobulin. The positive-selection--hybridization assay has been modified to facilitate the screening of large numbers of cDNA clones, and the modified assay should allow the isolation of cDNAs corresponding to any mRNA whose in vitro translation products can be immunoprecipitated. These modifications are of particular value in the isolation of cDNA clones corresponding to rare species of mRNA.

Citing Articles

Cellulase gene expression in ripening avocado fruit: The accumulation of cellulase mRNA and protein as demonstrated by cDNA hybridization and immunodetection.

Christoffersen R, Tucker M, Laties G Plant Mol Biol. 2013; 3(6):385-91.

PMID: 24310572 DOI: 10.1007/BF00033386.

Developmental expression of sunflower 11S storage protein genes.

Allen R, Nessler C, Thomas T Plant Mol Biol. 2013; 5(3):165-73.

PMID: 24306652 DOI: 10.1007/BF00015680.

Expression of alcohol dehydrogenase in rice embryos under anoxia.

Ricard B, Mocquot B, Fournier A, Delseny M, Pradet A Plant Mol Biol. 2013; 7(5):321-9.

PMID: 24302402 DOI: 10.1007/BF00032562.

Regulation of levels of nuclear transcripts for C4 photosynthesis in bundle sheath and mesophyll cells of maize leaves.

Sheen J, Bogorad L Plant Mol Biol. 2013; 8(3):227-38.

PMID: 24301127 DOI: 10.1007/BF00015031.

Differential expression of oxygen-evolving polypeptide genes in maize leaf cell types.

Sheen J, Sayre R, Bogorad L Plant Mol Biol. 2013; 9(3):217-26.

PMID: 24276970 DOI: 10.1007/BF00166458.

References

Schibler U, Marcu K, PERRY R . The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells. Cell. 1978; 15(4):1495-509. DOI: 10.1016/0092-8674(78)90072-7. View

Vitetta E, Capra J . The protein products of the murine 17th chromosome: genetics and structure. Adv Immunol. 1978; 26:147-93. DOI: 10.1016/s0065-2776(08)60230-8. View

Dagert M, Ehrlich S . Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979; 6(1):23-8. DOI: 10.1016/0378-1119(79)90082-9. View

Rogers J, Clarke P, Salser W . Sequence analysis of cloned cDNA encoding part of an immunoglobulin heavy chain. Nucleic Acids Res. 1979; 6(10):3305-21. PMC: 327935. DOI: 10.1093/nar/6.10.3305. View

Dobberstein B, Garoff H, Warren G, Robinson P . Cell-free synthesis and membrane insertion of mouse H-2Dd histocompatibility antigen and beta 2-microglobulin. Cell. 1979; 17(4):759-69. DOI: 10.1016/0092-8674(79)90316-7. View

Lingappa V, Cunningham B, Jazwinski S, Hopp T, Blobel G, Edelman G . Cell-free synthesis and segregation of beta 2-microglobulin. Proc Natl Acad Sci U S A. 1979; 76(8):3651-5. PMC: 383890. DOI: 10.1073/pnas.76.8.3651. View

Ricciardi R, Miller J, Roberts B . Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation. Proc Natl Acad Sci U S A. 1979; 76(10):4927-31. PMC: 413051. DOI: 10.1073/pnas.76.10.4927. View

Konkel D, Maizel Jr J, Leder P . The evolution and sequence comparison of two recently diverged mouse chromosomal beta--globin genes. Cell. 1979; 18(3):865-73. DOI: 10.1016/0092-8674(79)90138-7. View

Nagata S, Taira H, Hall A, Johnsrud L, Streuli M, Ecsodi J . Synthesis in E. coli of a polypeptide with human leukocyte interferon activity. Nature. 1980; 284(5754):316-20. DOI: 10.1038/284316a0. View

10.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

11.

Adams J, Gough N, Webb E, Tyler B, Jackson J, Cory S . Molecular cloning of mouse immunoglobulin heavy chain messenger ribonucleic acids coding for mu, alpha, gamma 1, gamma 2a, and gamma 3 chains. Biochemistry. 1980; 19(12):2711-9. DOI: 10.1021/bi00553a027. View

12.

Michaelson J, Rothenberg E, Boyse E . Genetic polymorphism of murine beta 2-microglobulin detected biochemically. Immunogenetics. 1980; 11(1):93-5. DOI: 10.1007/BF01567773. View

13.

Goodman R, Jacobs J, Chin W, Lund P, Dee P, Habener J . Nucleotide sequence of a cloned structural gene coding for a precursor of pancreatic somatostatin. Proc Natl Acad Sci U S A. 1980; 77(10):5869-73. PMC: 350173. DOI: 10.1073/pnas.77.10.5869. View

14.

Ploegh H, Orr H, Strominger J . Molecular cloning of a human histocompatibility antigen cDNA fragment. Proc Natl Acad Sci U S A. 1980; 77(10):6081-5. PMC: 350217. DOI: 10.1073/pnas.77.10.6081. View

15.

Gates 3rd F, Coligan J, Kindt T . Complete amino acid sequence of murine beta 2-microglobulin: structural evidence for strain-related polymorphism. Proc Natl Acad Sci U S A. 1981; 78(1):554-8. PMC: 319092. DOI: 10.1073/pnas.78.1.554. View

16.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

17.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

18.

Rabbitts T . Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA. Nature. 1976; 260(5548):221-5. DOI: 10.1038/260221a0. View

19.

Natori T, TANIGAKI N, Pressman D, Henriksen O, Appella E, Law L . The component fragments obtained by acid dissociation of papain-solubilized H-2 molecules. J Immunogenet. 1976; 3(1):35-47. DOI: 10.1111/j.1744-313x.1976.tb00554.x. View

20.

Natori T, TANIGAKI N, Pressman D . A mouse plasma substance carrying beta2-microglobulin activity and lacking in H-2 alloantigenic activity. J Immunogenet. 1976; 3(2):123-34. DOI: 10.1111/j.1744-313x.1976.tb00563.x. View