Localisation of a Series of Intra-RNA Cross-links in 16S RNA, Induced by Ultraviolet Irradiation of Escherichia Coli 30S Ribosomal Subunits

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1980 Jun 11

PMID 6160460

Citations 19

Authors

C Zwieb

R Brimacombe

Affiliations

Soon will be listed here.

Abstract

Mild ultraviolet irradiation of E. coli ribosomal subunits leads to the formation of a number of intra-RNA cross-links, in addition to the RNA-protein cross-links already reported (see refs. 9, 10). After partial ribonuclease digestion of the RNA from irradiated subunits, complexes containing these intra-RNA cross-links can be isolated on a two-dimensional gel electrophoresis system, and subjected to sequence analysis. A series of these cross-linked complexes is described, and the cross-linked RNA regions are compared with the secondary structures derived for 16S RNA (see refs. 6, 7).

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