Cloning and Expression of a Deoxyribonucleic Acid Fragment That Encodes for the Adhesive Antigen K99

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1980 Sep 1

PMID 6159326

Citations 23

Authors

J D van Embden

F K de Graaf

L M Schouls

J S Teppema

Affiliations

Soon will be listed here.

Abstract

Deoxyribonucleic acid fragments of the naturally occurring conjugative K99 plasmid were cloned into vectors pBR322 and pBR325. The smallest deoxyribonucleic acid segment obtained that still expressed K99 was 4.5 megadaltons in size. With regard to the serological, adhesive, and morphological properties, no differences in the nature of the K99 antigen was observed between Escherichia coli strains carrying recombinant plasmids and those carrying pRI9901. Furthermore, the regulation of K99 expression from the recombinant plasmid deoxyribonucleic acid was similar to that from pRI9901. the low level of K99 expression in E. coli K-12 compared with natural K99-producing isolates seems to be host specific.

Citing Articles

Chloroplast targeting of FanC, the major antigenic subunit of Escherichia coli K99 fimbriae, in transgenic soybean.

Garg R, Tolbert M, Oakes J, Clemente T, Bost K, Piller K Plant Cell Rep. 2007; 26(7):1011-23.

PMID: 17333019 DOI: 10.1007/s00299-007-0322-y.

Expression and immunogenicity of an Escherichia coli K99 fimbriae subunit antigen in soybean.

Piller K, Clemente T, Jun S, Petty C, Sato S, Pascual D Planta. 2004; 222(1):6-18.

PMID: 15609046 DOI: 10.1007/s00425-004-1445-9.

Analysis of a naturally occurring K99+ enterotoxigenic Escherichia coli strain that fails to produce K99.

Isaacson R, Patterson S Infect Immun. 1994; 62(10):4686-9.

PMID: 7927743 PMC: 303168. DOI: 10.1128/iai.62.10.4686-4689.1994.

Expression of the gene cluster associated with the Escherichia coli pilus adhesin K99.

Lee J, Isaacson R Infect Immun. 1995; 63(10):4143-9.

PMID: 7558331 PMC: 173582. DOI: 10.1128/iai.63.10.4143-4149.1995.

Purification, characterization, and partial covalent structure of Escherichia coli adhesive antigen K99.

de Graaf F, Klemm P, Gaastra W Infect Immun. 1981; 33(3):877-83.

PMID: 7026448 PMC: 350793. DOI: 10.1128/iai.33.3.877-883.1981.

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