In Vitro Adhesion of Escherichia Coli to Porcine Small Intestinal Epithelial Cells: Pili As Adhesive Factors

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1978 Aug 1

PMID 357285

Citations 63

Authors

R E Isaacson

P C Fusco

C C Brinton

H W Moon

Affiliations

Soon will be listed here.

Abstract

Escherichia coli strains with pili (K99 or 987P) known to facilitate intestinal colonization adhered in vitro to porcine intestinal epithelial cells. These strains adhered equally to both ileal and jejunal epithelial cells. A laboratory E. coli strain that has type 1 pili also adhered to porcine intestinal epithelial cells. When nonpiliated cells derived from 987P+, K99+, or type 1 pilus+ strains were used for in vitro adhesion assays, they failed to adhere. The attachment of piliated bacteria to epithelial cells was a saturable process that plateaued at 30 to 40 bacterial cells attached per epithelial cell. Competitive inhibition of bacterial cell attachment to epithelial cells with purified pili showed that only purified 987P competed against the 987P+ strain and only purified type 1 pili competed against the type 1 pilus+ strain. Competition between a K99+ strain and K99 was not consistently achieved. K99+, 987P+, and type 1 pilus+ bacteria could be prevented from adhering to epithelial cells by Fab fragments specific for K99, 987P, or type 1 pili, respectively. Fab fragments specific for non-K99 bacterial surface antigens did not inhibit adhesion of the K99+ strain. It is concluded that adhesion of E. coli to porcine intestinal epithelial cells in vitro is mediated by pili and that the epithelial cells used apparently had different receptors for different pili.

Citing Articles

Animal Enterotoxigenic Escherichia coli.

Dubreuil J, Isaacson R, Schifferli D EcoSal Plus. 2016; 7(1).

PMID: 27735786 PMC: 5123703. DOI: 10.1128/ecosalplus.ESP-0006-2016.

Reduction of enterotoxin induced fluid accumulation in ileal loops of neonatal calves with anti-F5 fimbriae recombinant antibody.

Sahagun-Ruiz A, Velazquez L, Bhaskaran S, Jay C, Morales-Salinas E, Rathore K Vet Res Commun. 2015; 39(4):229-36.

PMID: 26521056 DOI: 10.1007/s11259-015-9646-1.

Expression of anti-K99 scFv in transgenic rice tissues and its functional characterization.

Sunilkumar G, Waghela S, Campbell L, Rathore K Transgenic Res. 2008; 18(3):347-60.

PMID: 18830803 DOI: 10.1007/s11248-008-9223-2.

FimH forms catch bonds that are enhanced by mechanical force due to allosteric regulation.

Yakovenko O, Sharma S, Forero M, Tchesnokova V, Aprikian P, Kidd B J Biol Chem. 2008; 283(17):11596-605.

PMID: 18292092 PMC: 2431072. DOI: 10.1074/jbc.M707815200.

Escherichia coli strain RDEC-1 AF/R1 endogenous fimbrial glycoconjugate receptor molecules in rabbit small intestine.

Ryu H, Kim Y, Grange P, Cassels F Infect Immun. 2001; 69(2):640-9.

PMID: 11159950 PMC: 97934. DOI: 10.1128/IAI.69.2.640-649.2001.

References

Porter R . The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959; 73:119-26. PMC: 1197021. DOI: 10.1042/bj0730119. View

ORSKOV I, ORSKOV F, SOJKA W, LEACH J . Simultaneous occurrence of E. coli B and Lantigens in strains from diseased swine. Influence of cultivation temperature. Two new E. coli Kantigens: K 87 and K 88. Acta Pathol Microbiol Scand. 1961; 53:404-22. View

Isaacson R . K99 surface antigen of Escherichia coli: purification and partial characterization. Infect Immun. 1977; 15(1):272-9. PMC: 421358. DOI: 10.1128/iai.15.1.272-279.1977. View

Nagy B, Moon H, Isaacson R . Colonization of porcine intestine by enterotoxigenic Escherichia coli: selection of piliated forms in vivo, adhesion of piliated forms to epithelial cells in vitro, and incidence of a pilus antigen among porcine enteropathogenic E. coli. Infect Immun. 1977; 16(1):344-52. PMC: 421527. DOI: 10.1128/iai.16.1.344-352.1977. View

Isaacson R, Nagy B, Moon H . Colonization of porcine small intestine by Escherichia coli: colonization and adhesion factors of pig enteropathogens that lack K88. J Infect Dis. 1977; 135(4):531-9. DOI: 10.1093/infdis/135.4.531. View

SWANEY L, Liu Y, To C, To C, BRINTON Jr C . Isolation and characterization of Escherichia coli phase variants and mutants deficient in type 1 pilus production. J Bacteriol. 1977; 130(1):495-505. PMC: 235228. DOI: 10.1128/jb.130.1.495-505.1977. View

GUINEE P, Veldkamp J, Jansen W . Improved minca medium for the detection of K99 antigen in calf enterotoxigenic strains of Escherichia coli. Infect Immun. 1977; 15(2):676-8. PMC: 421422. DOI: 10.1128/iai.15.2.676-678.1977. View

Moon H, Nagy B, Isaacson R, ORSKOV I . Occurrence of K99 antigen on Escherichia coli isolated from pigs and colonization of pig ileum by K99+ enterotoxigenic E. coli from calves and pigs. Infect Immun. 1977; 15(2):614-20. PMC: 421411. DOI: 10.1128/iai.15.2.614-620.1977. View

Burrows M, Sellwood R, Gibbons R . Haemagglutinating and adhesive properties associated with the K99 antigen of bovine strains of Escherichia coli. J Gen Microbiol. 1976; 96(2):269-75. DOI: 10.1099/00221287-96-2-269. View

10.

Isaacson R, Konisky J . Studies of the regulation of colicin Ib synthesis. II. The synthesis of col Ib-P9 specific RNA in vivo. Mol Gen Genet. 1974; 132(3):223-32. DOI: 10.1007/BF00269395. View

11.

Jones G, Rutter J . Contribution of the K88 antigen of Escherichia coli to enteropathogenicity; protection against disease by neutralizing the adhesive properties of K88 antigen. Am J Clin Nutr. 1974; 27(12):1441-9. DOI: 10.1093/ajcn/27.12.1441. View

12.

Wilson M, Hohmann A . Immunity to Escherichia coli in pigs: adhesion of enteropathogenic Escherichia coli to isolated intestinal epithelial cells. Infect Immun. 1974; 10(4):776-82. PMC: 423022. DOI: 10.1128/iai.10.4.776-782.1974. View

13.

Jones G, Rutter J . Role of the K88 antigen in the pathogenesis of neonatal diarrhea caused by Escherichia coli in piglets. Infect Immun. 1972; 6(6):918-27. PMC: 419313. DOI: 10.1128/iai.6.6.918-927.1972. View

14.

Bertschinger H, Moon H, Whipp S . Association of Escherichia coli with the small intestinal epithelium. I. Comparison of enteropathogenic and nonenteropathogenic porcine strains in pigs. Infect Immun. 1972; 5(4):595-605. PMC: 422410. DOI: 10.1128/iai.5.4.595-605.1972. View

15.

Arbuckle J . The location of Escherichia coli in the pig intestine. J Med Microbiol. 1970; 3(2):333-40. DOI: 10.1099/00222615-3-2-333. View

16.

BRINTON Jr C . The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in gram negative bacteria. Trans N Y Acad Sci. 1965; 27(8):1003-54. DOI: 10.1111/j.2164-0947.1965.tb02342.x. View

17.

Hohmann A, Wilson M . Adherence of enteropathogenic Escherichia coli to intestinal epithelium in vivo. Infect Immun. 1975; 12(4):866-80. PMC: 415368. DOI: 10.1128/iai.12.4.866-880.1975. View

18.

Sellwood R, Gibbons R, Jones G, Rutter J . Adhesion of enteropathogenic Escherichia coli to pig intestinal brush borders: the existence of two pig phenotypes. J Med Microbiol. 1975; 8(3):405-11. DOI: 10.1099/00222615-8-3-405. View

19.

GUINEE P, Jansen W, Agterberg C . Detection of the K99 antigen by means of agglutination and immunoelectrophoresis in Escherichia coli isolates from calves and its correlation with entertoxigenicity. Infect Immun. 1976; 13(5):1369-77. PMC: 420767. DOI: 10.1128/iai.13.5.1369-1377.1976. View

20.

Nagy B, Moon H, Isaacson R . Colonization of porcine small intestine by Escherichia coli: ileal colonization and adhesion by pig enteropathogens that lack K88 antigen and by some acapsular mutants. Infect Immun. 1976; 13(4):1214-20. PMC: 420741. DOI: 10.1128/iai.13.4.1214-1220.1976. View