Timing of Swarmer Cell Cycle Morphogenesis and Macromolecular Synthesis by Hyphomicrobium Neptunium in Synchronous Culture

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1980 Oct 1

PMID 6158509

Citations 20

Authors

T M Wali

G R Hudson

D A Danald

R M Weiner

Affiliations

Soon will be listed here.

Abstract

The swarmer cycle of Hyphomicrobium neptunium consists of a temporal sequence of discrete developmental events. To time morphogenesis and to investigate modulations in macromolecular synthesis, we attempted methods for synchronous culture. During synchrony, swarmer maturation occurred over 32%, hyphal growth occurred over 36%, and bud maturation occurred over 32% of the time required to complete the swarmer cycle. Daughter cells were released after 265 min. Deoxyribonucleic acid replication was discontinuous, having a G1 period of approximately 180 min. In addition, ribonucleic acid and protein syntheses were depressed during the earlier phases of development.

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References

ROODYN D, Mandel H . A simple membrane fractionation method for determining the distribution of radioactivity in chemical fractions of Bacillus cereus. Biochim Biophys Acta. 1960; 41:80-8. DOI: 10.1016/0006-3002(60)90371-1. View

Hirsch P . Budding bacteria. Annu Rev Microbiol. 1974; 28(0):391-444. DOI: 10.1146/annurev.mi.28.100174.002135. View

Guerry P, Leblanc D, Falkow S . General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973; 116(2):1064-6. PMC: 285495. DOI: 10.1128/jb.116.2.1064-1066.1973. View

Meyers J, Sanchez D, ELWELL L, Falkow S . Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976; 127(3):1529-37. PMC: 232949. DOI: 10.1128/jb.127.3.1529-1537.1976. View

Weiner R, Blackman M . Inhibition of deoxyribonucleic acid synthesis and bud formation by nalidixic acid in Hyphomicrobium neptunium. J Bacteriol. 1973; 116(3):1398-404. PMC: 246499. DOI: 10.1128/jb.116.3.1398-1404.1973. View

Blackman M, Weiner R . Photomicrography of nalidixic acid treated Hyphomicrobium neptunium: inhibition of bud formation and bud separation. Can J Microbiol. 1975; 21(2):226-30. DOI: 10.1139/m75-032. View

Havenner J, McCardell B, Weiner R . Development of Defined, Minimal, and Complete Media for the Growth of Hyphomicrobium neptunium. Appl Environ Microbiol. 1979; 38(1):18-23. PMC: 243428. DOI: 10.1128/aem.38.1.18-23.1979. View

Leifson E . HYPHOMICROBIUM NEPTUNIUM SP. N. Antonie Van Leeuwenhoek. 1964; 30:249-56. DOI: 10.1007/BF02046730. View

WHITTENBURY R, Dow C . Morphogenesis and differentiation in Rhodomicrobium vannielii and other budding and prosthecate bacteria. Bacteriol Rev. 1977; 41(3):754-808. PMC: 414022. DOI: 10.1128/br.41.3.754-808.1977. View

10.

Moore R, Hirsch P . First generation synchrony of isolated Hyphomicrobium swarmer populations. J Bacteriol. 1973; 116(1):418-23. PMC: 246438. DOI: 10.1128/jb.116.1.418-423.1973. View

11.

Moore R, Hirsch P . Deoxyribonucleic acid base sequence homologies of some budding and prosthecate bacteria. J Bacteriol. 1972; 110(1):256-61. PMC: 247405. DOI: 10.1128/jb.110.1.256-261.1972. View

12.

Cooper S . A unifying model for the G1 period in prokaryotes and eukaryotes. Nature. 1979; 280(5717):17-9. DOI: 10.1038/280017a0. View

13.

Moore R, Hirsch P . Nuclear apparatus of Hyphomicrobium. J Bacteriol. 1973; 116(3):1447-55. PMC: 246504. DOI: 10.1128/jb.116.3.1447-1455.1973. View

14.

Powell D, ROBERSON B, Weiner R . Serological relationships among budding, prosthecate bacteria. Can J Microbiol. 1980; 26(2):209-7. DOI: 10.1139/m80-032. View

15.

Harder W, Attwood M . Biology, physiology and biochemistry of hyphomicrobia. Adv Microb Physiol. 1978; 17:303-59. DOI: 10.1016/s0065-2911(08)60060-0. View