Inhibition of Deoxyribonucleic Acid Synthesis and Bud Formation by Nalidixic Acid in Hyphomicrobium Neptunium

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1973 Dec 1

PMID 4127631

Citations 8

Authors

R M Weiner

M A Blackman

Affiliations

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Abstract

The relationship between chromosome replication and morphogenesis in the budding bacterium Hyphomicrobium neptunium has been investigated. Nalidixic acid was found to completely inhibit deoxyribonucleic acid synthesis, but not ribonucleic acid synthesis. The antibiotic was bacteriostatic to the organism for the initial 5 h of exposure; thereafter it was bacteriocidal. Observation of inhibited cultures revealed cells that had produced abnormally long stalks, but no buds. These results indicate that bud formation is coupled to chromosome replication in H. neptunium. They do not exclude the possibilities that cross wall formation and bud separation may also be coupled to chromosome replication.

Citing Articles

Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus.

Badger J, Hoover T, Brun Y, Weiner R, Laub M, Alexandre G J Bacteriol. 2006; 188(19):6841-50.

PMID: 16980487 PMC: 1595504. DOI: 10.1128/JB.00111-06.

Development of Defined, Minimal, and Complete Media for the Growth of Hyphomicrobium neptunium.

Havenner J, McCardell B, Weiner R Appl Environ Microbiol. 1979; 38(1):18-23.

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Generation of buds, swellings, and branches instead of filaments after blocking the cell cycle of Rhizobium meliloti.

Latch J, Margolin W J Bacteriol. 1997; 179(7):2373-81.

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Fine-structure evidence for cell membrane partitioning of the nucleoid and cytoplasm during bud formation in Hyphomonas species.

Zerfas P, Kessel M, Quintero E, Weiner R J Bacteriol. 1997; 179(1):148-56.

PMID: 8981992 PMC: 178673. DOI: 10.1128/jb.179.1.148-156.1997.

Trimethoprim enhances the antibacterial activity of nalidixic and oxolinic acids and delays the emergence of resistance.

Bertolini A, Castelli M, Genedani S, Garuti M Experientia. 1980; 36(2):243-4.

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