Inhibition of Polymorphonuclear Leukocyte Chemiluminescence for Detection of Immune Complexes in Human Sera

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1980 Sep 1

PMID 6156953

Citations 5

Authors

N J Doll

M R Wilson

J E Salvaggio

Affiliations

Soon will be listed here.

Abstract

An assay for the detection and quantitation of immune complexes is described. Experimental immune complexes or aggregated human gamma globulin (AHG) were incubated with polymorphonuclear leukocytes (PMN). After challenge of the PMN with opsonized zymosan, chemiluminescence was recorded in a scintillation spectrometer. A quantitative inhibition of chemiluminescence could be demonstrated by the interaction of PMN with immune complexes or AHG. Experimental immune complexes of bovine serum albumin-anti-bovine serum albumin were formed and tested by this assay, and immune complexes formed near antigen excess were best described by this technique. The technique was used to demonstrate immune complexes in the sera from patients with systemic lupus erythematosus, rheumatoid arthritis, and vasculitis. Immune complexes were quantitated by reference to a standard curve using AHG. By this technique, normal human sera had < 10 micrograms of AHG per milliliter of serum. Immune complexes at levels above this were detected in 9/15 patients with systemic lupus erythematosus, 18/30 patients with rheumatoid arthritis, and 2/5 patients with vasculitis. Therefore, this assay is a sensitive, simple method for measurement of circulating immune complexes in the sera of patients with certain connective tissue diseases.

Citing Articles

Direct activation of neutrophil chemiluminescence by rheumatoid sera and synovial fluid.

Gale R, Bertouch J, Bradley J, Roberts-Thomson P Ann Rheum Dis. 1983; 42(2):158-62.

PMID: 6847260 PMC: 1001091. DOI: 10.1136/ard.42.2.158.

Effect of glomerular basement membrane on the initiation of chemiluminescence and lysosomal enzyme release in human polymorphonuclear leucocytes: an in vitro model of glomerular disease.

Davies M, Coles G, Harber M Immunology. 1984; 52(1):151-9.

PMID: 6715018 PMC: 1454578.

Neutrophil activation by immune complexes and the role of rheumatoid factor.

Gale R, Bertouch J, Gordon T, Bradley J, Roberts-Thomson P Ann Rheum Dis. 1984; 43(1):34-9.

PMID: 6696514 PMC: 1001214. DOI: 10.1136/ard.43.1.34.

Differential generation of chemiluminescence--detectable oxygen radicals by normal polymorphonuclear leukocytes challenged with sera from systemic lupus erythematosus and rheumatoid arthritis patients.

Donofrio C, Maly F, Fischer H, MAAS D Klin Wochenschr. 1984; 62(15):710-6.

PMID: 6492692 DOI: 10.1007/BF01725703.

Joint fluid leukocyte activation by preformed immune complexes.

Thomsen P, Bjursten L, Ericson L Inflammation. 1986; 10(3):243-56.

PMID: 3744475 DOI: 10.1007/BF00916120.

References

Faber V, Elling P . Leucocyte-specific anti-nuclear factors in patients with felty's syndrome, rheumatoid arthritis, systemic lupus erythematosus and other diseases. Acta Med Scand. 1966; 179(3):257-67. DOI: 10.1111/j.0954-6820.1966.tb05457.x. View

Agnello V, Winchester R, KUNKEL H . Precipitin reactions of the C1q component of complement with aggregated gamma-globulin and immune complexes in gel diffusion. Immunology. 1970; 19(6):909-19. PMC: 1455666. View

Henson P . Interaction of cells with immune complexes: adherence, release of constituents, and tissue injury. J Exp Med. 1971; 134(3 Pt 2):114s-135s. View

Weissmann G, Zurier R, Spieler P, Goldstein I . Mechanisms of lysosomal enzyme release from leukocytes exposed to immune complexes and other particles. J Exp Med. 1971; 134(3 Pt 2):149s-165s. View

Agnello V, Koffler D, Eisenberg J, Winchester R, Kundel H . C1g precipitins in the sera of patients with systemic lupus erythematosus and other hypocomplementemic states: characterization of high and low molecular weight types. J Exp Med. 1971; 134(3 Pt 2):228s-241s. View

Winchester R, KUNKEL H, Agnello V . Occurrence of -globulin complexes in serum and joint fluid of rheumatoid arthritis patients: use of monoclonal rheumatoid factors as reagents for their demonstration. J Exp Med. 1971; 134(3 Pt 2):286s-295s. View

Henson P . The immunologic release of constituents from neutrophil leukocytes. II. Mechanisms of release during phagocytosis, and adherence to nonphagocytosable surfaces. J Immunol. 1971; 107(6):1547-57. View

Henson P, Johnson H, Spiegelberg H . The release of granule enzymes from human neutrophils stimulated by aggregated immunoglobulins of different classes and subclasses. J Immunol. 1972; 109(6):1182-92. View

Nydegger U, Lambert P, Gerber H, MIESCHER P . Circulating immune complexes in the serum in systemic lupus erythematosus and in carriers of hepatitis B antigen. Quantitation by binding to radiolabeled C1q. J Clin Invest. 1974; 54(2):297-309. PMC: 301557. DOI: 10.1172/JCI107765. View

10.

Goldstein I, Feit F, Weissmann G . Enhancement of nitroblue tetrazolium dye reduction by leukocytes exposed to a component of complement in the absence of phagocytosis. J Immunol. 1975; 114(1 Pt 2):516-8. View

11.

Luthra H, MCDUFFIE F, Hunder G, Samayoa E . Immune complexes in sera and synovial fluids of patients with rheumatoid arthritis. Radioimmunoassay with monocylonal rheumatoid factor. J Clin Invest. 1975; 56(2):458-66. PMC: 436606. DOI: 10.1172/JCI108112. View

12.

Mantovani B . Different roles of IgG and complement receptors in phagocytosis by polymorphonuclear leukocytes. J Immunol. 1975; 115(1):15-7. View

13.

Henson P, Oades Z . Stimulation of human neutrophils by soluble and insoluble immunoglobulin aggregates. Secretion of granule constituents and increased oxidation of glucose. J Clin Invest. 1975; 56(4):1053-61. PMC: 301961. DOI: 10.1172/JCI108152. View

14.

Theofilopoulos A, Wilson C, Dixon F . The Raji cell radioimmune assay for detecting immune complexes in human sera. J Clin Invest. 1976; 57(1):169-82. PMC: 436637. DOI: 10.1172/JCI108257. View

15.

Scribner D, FAHRNEY D . Neutrophil receptors for IgG and complement: their roles in the attachment and ingestion phases of phagocytosis. J Immunol. 1976; 116(4):892-7. View

16.

JOHNSTON Jr R, Lehmeyer J . Elaboration of toxic oxygen by-products by neutrophils in a model of immune complex disease. J Clin Invest. 1976; 57(4):836-41. PMC: 436726. DOI: 10.1172/JCI108359. View

17.

Goldstein I, Kaplan H, Radin A, Frosch M . Independent effects of IgG and complement upon human polymorphonuclear leukocyte function. J Immunol. 1976; 117(4):1282-7. View

18.

Allen R . Evaluation of serum opsonic capacity by quantitating the initial chemiluminescent response from phagocytizing polymorphonuclear leukocytes. Infect Immun. 1977; 15(3):828-33. PMC: 421447. DOI: 10.1128/iai.15.3.828-833.1977. View

19.

Steffelaar J, Ten Kate F, Nap M, Swaak A, De Graaffreitsma C, van Elven E . Immune complex detection by immunofluorescence on polymorphonuclear leucocytes. Clin Exp Immunol. 1977; 27(3):391-6. PMC: 1540934. View

20.

Allen R, STJERNHOLM R, Reed M, Harper 3rd T, Gupta S, Steele R . Correlation of metabolic and chemiluminescent responses of granulocytes from three female siblings with chronic granulomatous disease. J Infect Dis. 1977; 136(4):510-8. DOI: 10.1093/infdis/136.4.510. View