Alpha 2.0
Login Register
» Articles » PMID: 6154247

Effects of PGI2 and PGI Analogues on CAMP Levels in Cultured Endothelial and Smooth Muscle Cells Derived from Bovine Arteries

Overview
Journal Naunyn Schmiedebergs Arch Pharmacol
Specialty Pharmacology
Date 1980 Feb 1
PMID 6154247
Citations 8
Authors
A Dembinska-Kiec
W Rucker
P S Schonhofer
Affiliations
Soon will be listed here.
Abstract

PGI2 increased cAMP levels in cultured endothelial cells derived from bovine aortas in the presence of isobutylmethylxanthine (IBMX). Stable PGI analogues were more effective, while PGE1 was without effect. In cultured smooth muscle cells from bovine aortas, PGI2, pGE1 and the stable PGI analogues dose-dependently increased cAMP levels in the absence and presence of IBMX. The data confirm the stimulatory effect of PGI2 on cAMP content in bovine coronary artery rings and suggest that the effect is mainly due to the stimulatory action of PGI2 on cAMP in smooth muscle cells.

Citing Articles

Effects of prostacyclin (PGI2) and adenosine (ASN) on total and regional blood flow of isolated, collateralized dog hearts.

SCHOLTHOLT J, Birringer H, Fiedler V, Scholkens B Basic Res Cardiol. 1981; 76(3):313-27.

PMID: 7023464 DOI: 10.1007/BF01907775.

Effects of vasoactive and inflammatory agents on cyclic AMP levels in W138 fibroblasts, endothelial and vascular smooth muscle cells in culture.

Ager A, Gordon J Agents Actions. 1980; 10(6):569-72.

PMID: 6267917 DOI: 10.1007/BF02024169.

Relationship between prostacyclin biosynthesis and cyclic AMP in cultured rabbit mesothelial cells.

Van de Velde V, Bult H, Herman A Naunyn Schmiedebergs Arch Pharmacol. 1983; 322(3):245-8.

PMID: 6191226 DOI: 10.1007/BF00500772.

Endothelium-mediated coronary dilatation by adenosine does not depend on endothelial adenylate cyclase activation: studies in isolated guinea pig hearts.

Newman W, Becker B, Heier M, Nees S, Gerlach E Pflugers Arch. 1988; 413(1):1-7.

PMID: 2464158 DOI: 10.1007/BF00581221.

Pig aortic endothelial-cell cyclic nucleotide phosphodiesterases. Use of phosphodiesterase inhibitors to evaluate their roles in regulating cyclic nucleotide levels in intact cells.

Souness J, Diocee B, Martin W, Moodie S Biochem J. 1990; 266(1):127-32.

PMID: 2155604 PMC: 1131105. DOI: 10.1042/bj2660127.

References
1.
Dembinska-Kiec A, Rucker W, Schonhofer P, Gandolfi C . Prostacyclin analogs: antiaggregatory potency and enhancement of cAMP levels in human platelet rich plasma. Thromb Haemost. 1979; 42(4):1340-3. View
2.
Schror K, Rosen P . Prostacyclin (PGI2) decreases the cyclic AMP level in coronary arteries. Naunyn Schmiedebergs Arch Pharmacol. 1979; 306(1):101-3. DOI: 10.1007/BF00515602. View
3.
Dembinska-Kiec A, Rucker W, Schonhofer P . PGI2 enhanced cAMP content in bovine coronary arteries in the presence of isobutylmethylxanthine. Naunyn Schmiedebergs Arch Pharmacol. 1979; 308(2):107-10. DOI: 10.1007/BF00499051. View
4.
Macarak E, Howard B, Kefalides N . Properties of calf endothelial cells in culture. Lab Invest. 1977; 36(1):62-7. View
5.
Moncada S, Herman A, Higgs E, Vane J . Differential formation of prostacyclin (PGX or PGI2) by layers of the arterial wall. An explanation for the anti-thrombotic properties of vascular endothelium. Thromb Res. 1977; 11(3):323-44. DOI: 10.1016/0049-3848(77)90185-2. View