Ontogenetic Appearance of Somatostatin-containing Nerve Terminals in the Median Eminence of Rats

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1984 Jan 1

PMID 6143619

Citations 4

Authors

T Adachi

M Ohtsuka

S Hisano

Y Tsuruo

S Daikoku

Affiliations

Soon will be listed here.

Abstract

The development of immunoreactive (ir) somatostatin-containing nerve terminals in the rat median eminence (ME) has been examined electron-microscopically. Nerve fibers containing ir particles scattered throughout the axoplasm are first seen in the external layer of the ME on day 18.5 of gestation, and, on day 21.5 appear to terminate on the basement membrane of the perivascular space of the portal vessels. After birth, the fiber terminals contain several membrane-limited granules, which are labeled with ir PAP particles. Ultrathin, Epon-embedded sections of ME, treated by the protein A gold-labeling method for somatostatin, demonstrate positively labeled granules in the nerve fibers in the postnatal ME, but in the prenatal tissue, no specific gold-labeling is found. These findings show that, in the external layer of the ME, somatostatin storing occurs in the granules in the axonal terminals after birth.

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