Immunocytochemical Localization of Prosomatostatin Fragments in Maturing and Mature Secretory Granules of Pancreatic and Gastrointestinal D Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1983 Jan 1

PMID 6130530

Citations 11

Authors

M Ravazzola

R Benoit

N Ling

R Guillemin

L Orci

Affiliations

Soon will be listed here.

Abstract

Pancreatic and gastrointestinal D cells were examined by immunocytochemistry using antisera against somatostatin-28 (SS28) and its NH2-terminal fragment SS28-(1-12), followed by the staphylococcal protein A-gold (pAg) complex. In pancreatic and gastric D cells incubated with antiserum against SS28-(1-12) the gold particles produced intense staining of the mature secretory granules but weaker staining of the immature granules associated with the Golgi area, whereas after SS28 antiserum treatment the particles accumulated selectively over the population of immature secretory granules. In intestinal D cells not only SS28-(1-12) but also SS28 antiserum produced an intense gold staining over the mature delta granules. These observations show that the relative amounts of immunoreactive sites related to SS28 and its cleavage product SS28-(1-12) in maturing and mature secretory granules are different in pancreatic, gastric, and intestinal D cells.

Citing Articles

Paracrine signaling in islet function and survival.

Hartig S, Cox A J Mol Med (Berl). 2020; 98(4):451-467.

PMID: 32067063 PMC: 7899133. DOI: 10.1007/s00109-020-01887-x.

Expression of Secretogranin III in Chicken Endocrine Cells: Its Relevance to the Secretory Granule Properties of Peptide Prohormone Processing and Bioactive Amine Content.

Gomi H, Morikawa S, Shinmura N, Moki H, Yasui T, Tsukise A J Histochem Cytochem. 2015; 63(5):350-66.

PMID: 25673289 PMC: 4409946. DOI: 10.1369/0022155415575032.

Biological redundancy of endogenous GPCR ligands in the gut and the potential for endogenous functional selectivity.

Thompson G, Canals M, Poole D Front Pharmacol. 2014; 5:262.

PMID: 25506328 PMC: 4246669. DOI: 10.3389/fphar.2014.00262.

Pathways involved in targeting and secretion of a lysosomal enzyme in Dictyostelium discoideum.

Mierendorf Jr R, Cardelli J, Dimond R J Cell Biol. 1985; 100(5):1777-87.

PMID: 3988807 PMC: 2113895. DOI: 10.1083/jcb.100.5.1777.

Somatostatin immunoreactivity in the cat adrenal medulla. Localization and characterization.

Vincent S, McIntosh C, Reiner P, Brown J Histochemistry. 1987; 87(5):483-6.

PMID: 2892812 DOI: 10.1007/BF00496821.

References

LIKE A, Orci L . Embryogenesis of the human pancreatic islets: a light and electron microscopic study. Diabetes. 1972; 21(2 Suppl):511-34. DOI: 10.2337/diab.21.2.s511. View

BRAZEAU P, Vale W, Burgus R, Ling N, Butcher M, Rivier J . Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science. 1973; 179(4068):77-9. DOI: 10.1126/science.179.4068.77. View

Hokfelt T, Efendic S, Johansson O, Luft R, Arimura A . Immunohistochemical localization of somatostatin (growth hormone release-inhibiting factor) in the guinea pig brain. Brain Res. 1974; 80(1):165-9. DOI: 10.1016/0006-8993(74)90737-9. View

Luft R, Efendic S, Hokfelt T, Johansson O, Arimura A . Immunohistochemical evidence for the localization of somatostatin--like immunoreactivity in a cell population of the pancreatic islets. Med Biol. 1974; 52(6):428-30. View

Dubois M . Immunoreactive somatostatin is present in discrete cells of the endocrine pancreas. Proc Natl Acad Sci U S A. 1975; 72(4):1340-3. PMC: 432529. DOI: 10.1073/pnas.72.4.1340. View

Polak J, Pearse A, Grimelius L, Bloom S . Growth-hormone release-inhibiting hormone in gastrointestinal and pancreatic D cells. Lancet. 1975; 1(7918):1220-2. DOI: 10.1016/s0140-6736(75)92198-4. View

Dubois M, Barry J, Leonardelli J . [Immunofluorescence demonstration and distribution of somatostatin (SRIF) in the median eminence of vertebrates (mammals, birds, amphibia, fishes)]. C R Acad Hebd Seances Acad Sci D. 1974; 279(25):1899-902. View

Pelletier G, LECLERC R, Arimura A, Schally A . Letter: Immunohistochemical localization of somatostatin in the rat pancreas. J Histochem Cytochem. 1975; 23(9):699-702. DOI: 10.1177/23.9.1100709. View

Orci L, Baetens D, Dubois M, Rufener C . Evidence for the D-cell of the pancreas secreting somatostatin. Horm Metab Res. 1975; 7(5):400-2. DOI: 10.1055/s-0028-1093736. View

10.

Rufener C, Dubois M, Malaisse Lagae F, Oric L . Immuno-fluorescent reactivity to anti-somatostatin in the gastro-intestinal mucosa of the dog. Diabetologia. 1975; 11(4):321-4. DOI: 10.1007/BF00422398. View

11.

Arimura A, Sato H, Dupont A, Nishi N, Schally A . Somatostatin: abundance of immunoreactive hormone in rat stomach and pancreas. Science. 1975; 189(4207):1007-9. DOI: 10.1126/science.56779. View

12.

Conlon J, Zyznar E, Vale W, Unger R . Multiple forms of somatostatin-like immunoreactivity in canine pancreas. FEBS Lett. 1978; 94(2):327-30. DOI: 10.1016/0014-5793(78)80968-5. View

13.

Roth J, Bendayan M, Orci L . Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J Histochem Cytochem. 1978; 26(12):1074-81. DOI: 10.1177/26.12.366014. View

14.

Patel Y, Reichlin S . Somatostatin in hypothalamus, extrahypothalamic brain, and peripheral tissues of the rat. Endocrinology. 1978; 102(2):523-30. DOI: 10.1210/endo-102-2-523. View

15.

Noe B, Fletcher D, BAUER G, Weir G, Patel Y . Somatostatin biosynthesis occurs in pancreatic islets. Endocrinology. 1978; 102(6):1675-85. DOI: 10.1210/endo-102-6-1675. View

16.

Rorstad O, Epelbaum J, BRAZEAU P, Martin J . Chromatographic and biological properties of immunoreactive somatostatin in hypothalamic and extrahypothalamic brain regions of the rat. Endocrinology. 1979; 105(5):1083-92. DOI: 10.1210/endo-105-5-1083. View

17.

Zyznar E, Conlon J, Schusdziarra V, Unger R . Properties of somatostatin-like immunoreactive polypeptides in the canine extrahypothalamic brain and stomach. Endocrinology. 1979; 105(6):1426-31. DOI: 10.1210/endo-105-6-1426. View

18.

Zingg H, Patel Y . Somatostatin precursors: evidence for presence in and release from rat median eminence and neurohypophysis. Biochem Biophys Res Commun. 1979; 90(2):466-72. DOI: 10.1016/0006-291x(79)91258-0. View

19.

Lauber M, CAMIER M, Cohen P . Higher molecular weight forms of immunoreactive somatostatin in mouse hypothalamic extracts: evidence of processing in vitro. Proc Natl Acad Sci U S A. 1979; 76(11):6004-8. PMC: 411782. DOI: 10.1073/pnas.76.11.6004. View

20.

Pradayrol L, Jornvall H, Mutt V, Ribet A . N-terminally extended somatostatin: the primary structure of somatostatin-28. FEBS Lett. 1980; 109(1):55-8. DOI: 10.1016/0014-5793(80)81310-x. View