Molecular Cloning and Genetic Mapping of the PET494 Gene of Saccharomyces Cerevisiae

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1984 Jan 1

PMID 6092852

Citations 12

Authors

P P Muller

T D Fox

Affiliations

Soon will be listed here.

Abstract

The activity of the nuclear gene PET494 is required to allow expression of the yeast mitochondrial gene oxi2. To aid the study of the mechanism of action of PET494 we have isolated this gene from yeast DNA. A clone bank of yeast DNA fragments in a yeast-E. coli shuttle vector was screened by transformation for a plasmid able to complement the pet494-1 amber mutation. A complementing plasmid was obtained that contained a unique 4.4 kb yeast sequence. This 4.4 kb sequence contains the PET494 gene. Integration of a plasmid containing it into chromosomal DNA by homologous recombination, and subsequent genetic analysis, demonstrated that the 4.4 kb fragment was tightly linked to the pet494-1 mutation. In addition, the corresponding 4.4 kb sequence isolated from a pet494-1 mutant failed to complement the mutation. A 2 kb fragment, subcloned from the original plasmid retained the ability to complement the mutation. The pet494-1 mutation maps to chromosome XIV between rna2 and lys9, approximately 2.4 cm from lys9.

Citing Articles

Highly diverged homologs of Saccharomyces cerevisiae mitochondrial mRNA-specific translational activators have orthologous functions in other budding yeasts.

Costanzo M, Bonnefoy N, Williams E, Clark-Walker G, Fox T Genetics. 2000; 154(3):999-1012.

PMID: 10757749 PMC: 1460983. DOI: 10.1093/genetics/154.3.999.

Suppression of a defect in the 5' untranslated leader of mitochondrial COX3 mRNA by a mutation affecting an mRNA-specific translational activator protein.

Costanzo M, Fox T Mol Cell Biol. 1993; 13(8):4806-13.

PMID: 8393138 PMC: 360107. DOI: 10.1128/mcb.13.8.4806-4813.1993.

Product of Saccharomyces cerevisiae nuclear gene PET494 activates translation of a specific mitochondrial mRNA.

Costanzo M, Fox T Mol Cell Biol. 1986; 6(11):3694-703.

PMID: 3099165 PMC: 367130. DOI: 10.1128/mcb.6.11.3694-3703.1986.

Transformation of yeast by agitation with glass beads.

Costanzo M, Fox T Genetics. 1988; 120(3):667-70.

PMID: 3066683 PMC: 1203545. DOI: 10.1093/genetics/120.3.667.

PET111, a Saccharomyces cerevisiae nuclear gene required for translation of the mitochondrial mRNA encoding cytochrome c oxidase subunit II.

Poutre C, Fox T Genetics. 1987; 115(4):637-47.

PMID: 3034718 PMC: 1203096. DOI: 10.1093/genetics/115.4.637.

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