Transformation in Escherichia Coli: Cryogenic Preservation of Competent Cells

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1977 Oct 1

PMID 334731

Citations 103

Authors

D A Morrison

Affiliations

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Abstract

Escherichia coli cells prepared for transformation by treatment with cold 0.1 M CaCl2 remained viable and competent after storage at -82 degrees C in 15% glycerol; thawed-cell samples yielded up to 10(6) transformants per microgram of plasmid deoxyribonucleic acid.

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