Analysis of Immunoglobulin Heavy Chain V-region Genes Belonging to the V NP-gene Family

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1984 Sep 11

PMID 6091043

Citations 8

Authors

T Blankenstein

G Zoebelein

U Krawinkel

Affiliations

Soon will be listed here.

Abstract

A method was devised to clone immunoglobulin VH-region genes located on selected restriction fragments from genomic DNA directly into M13 vectors for subsequent nucleotide sequence analysis. Ten recombinant M13 clones representing four so far unknown VH-region genes of the VNP-gene family have been analysed. Sequence comparison shows that these genes are closely related to other VH-genes of the VNP gene family. One of the VH-genes exhibits a so far unobserved unusual length of 100 2/3 codons and appears to be functional. Analysis of the variation of the isolated VH-genes suggests that framework and complementarity determining regions are exposed to separate types of selective pressures.

Citing Articles

Immunoglobulin VH gene sequence analysis of spontaneous murine immunoglobulin-secreting B-cell tumours with clinical features of human disease.

Zhu D, van ARKEL C, King C, Meirvenne S, de Greef C, Thielemans K Immunology. 1998; 93(2):162-70.

PMID: 9616364 PMC: 1364174. DOI: 10.1046/j.1365-2567.1998.00428.x.

B cell function in mice transgenic for mCTLA4-H gamma 1: lack of germinal centers correlated with poor affinity maturation and class switching despite normal priming of CD4+ T cells.

Lane P, Burdet C, Hubele S, Scheidegger D, Muller U, McConnell F J Exp Med. 1994; 179(3):819-30.

PMID: 7509361 PMC: 2191407. DOI: 10.1084/jem.179.3.819.

Functional and pseudogenes are similarly organized and may equally contribute to the extensive antibody diversity of the IgVHII family.

Schiff C, Milili M, Fougereau M EMBO J. 1985; 4(5):1225-30.

PMID: 3924600 PMC: 554328. DOI: 10.1002/j.1460-2075.1985.tb03764.x.

Members of novel VH gene families are found in VDJ regions of polyclonally activated B-lymphocytes.

Winter E, Radbruch A, Krawinkel U EMBO J. 1985; 4(11):2861-7.

PMID: 2998759 PMC: 554590. DOI: 10.1002/j.1460-2075.1985.tb04015.x.

Dynamic gene interactions in the evolution of rabbit VH genes: a four codon duplication and block homologies provide evidence for intergenic exchange.

McCormack W, Laster S, Marzluff W, Roux K Nucleic Acids Res. 1985; 13(19):7041-54.

PMID: 2997735 PMC: 322021. DOI: 10.1093/nar/13.19.7041.

References

Benton W, Davis R . Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977; 196(4286):180-2. DOI: 10.1126/science.322279. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Reth M, Hammerling G, Rajewsky K . Analysis of the repertoire of anti-NP antibodies in C57BL/6 mice by cell fusion. I. Characterization of antibody families in the primary and hyperimmune response. Eur J Immunol. 1978; 8(6):393-400. DOI: 10.1002/eji.1830080605. View

Bernard O, Hozumi N, Tonegawa S . Sequences of mouse immunoglobulin light chain genes before and after somatic changes. Cell. 1978; 15(4):1133-44. DOI: 10.1016/0092-8674(78)90041-7. View

Sakano H, Huppi K, Heinrich G, Tonegawa S . Sequences at the somatic recombination sites of immunoglobulin light-chain genes. Nature. 1979; 280(5720):288-94. DOI: 10.1038/280288a0. View

Max E, Seidman J, Leder P . Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene. Proc Natl Acad Sci U S A. 1979; 76(7):3450-4. PMC: 383843. DOI: 10.1073/pnas.76.7.3450. View

Lomedico P, Rosenthal N, Efstratidadis A, Gilbert W, Kolodner R, Tizard R . The structure and evolution of the two nonallelic rat preproinsulin genes. Cell. 1979; 18(2):545-58. DOI: 10.1016/0092-8674(79)90071-0. View

Schilling J, Clevinger B, Davie J, Hood L . Amino acid sequence of homogeneous antibodies to dextran and DNA rearrangements in heavy chain V-region gene segments. Nature. 1980; 283(5742):35-40. DOI: 10.1038/283035a0. View

Weigert M, Perry R, Kelley D, Hunkapiller T, Schilling J, Hood L . The joining of V and J gene segments creates antibody diversity. Nature. 1980; 283(5746):497-9. DOI: 10.1038/283497a0. View

10.

Clevinger B, Schilling J, Hood L, Davie J . Structural correlates of cross-reactive and individual idiotypic determinants on murine antibodies to alpha-(1 leads to 3) dextran. J Exp Med. 1980; 151(5):1059-70. PMC: 2185843. DOI: 10.1084/jem.151.5.1059. View

11.

Early P, Huang H, Davis M, Calame K, Hood L . An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH. Cell. 1980; 19(4):981-92. DOI: 10.1016/0092-8674(80)90089-6. View

12.

Sakano H, Maki R, Kurosawa Y, Roeder W, Tonegawa S . Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes. Nature. 1980; 286(5774):676-83. DOI: 10.1038/286676a0. View

13.

SANGER F, Coulson A, Barrell B, Smith A, Roe B . Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980; 143(2):161-78. DOI: 10.1016/0022-2836(80)90196-5. View

14.

Gearhart P, Johnson N, Douglas R, Hood L . IgG antibodies to phosphorylcholine exhibit more diversity than their IgM counterparts. Nature. 1981; 291(5810):29-34. DOI: 10.1038/291029a0. View

15.

Gough N, Bernard O . Sequences of the joining region genes for immunoglobulin heavy chains and their role in generation of antibody diversity. Proc Natl Acad Sci U S A. 1981; 78(1):509-13. PMC: 319083. DOI: 10.1073/pnas.78.1.509. View

16.

Bothwell A, Paskind M, Reth M, Rajewsky K, Baltimore D . Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a gamma 2a variable region. Cell. 1981; 24(3):625-37. DOI: 10.1016/0092-8674(81)90089-1. View

17.

Givol D, Zakut R, Effron K, Rechavi G, Ram D, Cohen J . Diversity of germ-line immunoglobulin VH genes. Nature. 1981; 292(5822):426-30. DOI: 10.1038/292426a0. View

18.

Crews S, Griffin J, Huang H, Calame K, Hood L . A single VH gene segment encodes the immune response to phosphorylcholine: somatic mutation is correlated with the class of the antibody. Cell. 1981; 25(1):59-66. DOI: 10.1016/0092-8674(81)90231-2. View

19.

Cohen J, Effron K, Rechavi G, Ben-Neriah Y, Zakut R, Givol D . Simple DNA sequences in homologous flanking regions near immunoglobulin VH genes: a role in gene interaction?. Nucleic Acids Res. 1982; 10(11):3353-70. PMC: 320716. DOI: 10.1093/nar/10.11.3353. View

20.

Alt F, Baltimore D . Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions. Proc Natl Acad Sci U S A. 1982; 79(13):4118-22. PMC: 346588. DOI: 10.1073/pnas.79.13.4118. View