Identification of a Ribosome-binding Site for a Leader Peptide Encoded by Rous Sarcoma Virus RNA

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1984 Sep 1

PMID 6088795

Citations 16

Authors

R B Petersen

C H Hensel

P B Hackett

Affiliations

Soon will be listed here.

Abstract

A new method for identifying ribosome-binding sites was developed to determine whether AUG codons in the 5'-terminal RNA sequence of Rous sarcoma virus were used to initiate protein synthesis. We found that when translation is inhibited, the major ribosome-binding site on Rous sarcoma virus RNA is at the 5'-proximal AUG codon, even though the primary translational product from this RNA, Pr76gag, is encoded behind the fourth AUG codon 331 bases downstream from the observed initiation site. These results suggest that ribosomes can initiate translation on Rous sarcoma virus RNA at more than one site, thereby producing a seven-amino-acid peptide, as well as the gag gene polyprotein precursor of Mr 76,000.

Citing Articles

The structure and function of the rous sarcoma virus RNA stability element.

Withers J, Beemon K J Cell Biochem. 2011; 112(11):3085-92.

PMID: 21769913 PMC: 3810391. DOI: 10.1002/jcb.23272.

Rous sarcoma virus translation revisited: characterization of an internal ribosome entry segment in the 5' leader of the genomic RNA.

Deffaud C, Darlix J J Virol. 2000; 74(24):11581-8.

PMID: 11090156 PMC: 112439. DOI: 10.1128/jvi.74.24.11581-11588.2000.

Autogenous regulation of RNA translation and packaging by Rous sarcoma virus Pr76gag.

Sonstegard T, Hackett P J Virol. 1996; 70(10):6642-52.

PMID: 8794299 PMC: 190705.

Effects of the open reading frames in the Rous sarcoma virus leader RNA on translation.

Moustakas A, Sonstegard T, Hackett P J Virol. 1993; 67(7):4350-7.

PMID: 8389931 PMC: 237805. DOI: 10.1128/JVI.67.7.4350-4357.1993.

The first and third uORFs in RSV leader RNA are efficiently translated: implications for translational regulation and viral RNA packaging.

Donze O, Damay P, SPAHR P Nucleic Acids Res. 1995; 23(5):861-8.

PMID: 7708504 PMC: 306771. DOI: 10.1093/nar/23.5.861.

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