High [K+] Alters the Stimulus-hydrosmotic Response Coupling in Toad Bladder

Overview

Journal Pflugers Arch

Specialty Physiology

Date 1984 Apr 1

PMID 6087265

Citations 3

Authors

A Grosso

R C de Sousa

Affiliations

Soon will be listed here.

Abstract

Substitution of K+ for Na+ in the Ringer solution bathing the inner surface of toad urinary bladders (Bufo marinus) had no effect on basal water permeability but significantly altered the stimulus-hydrosmotic response of this epithelium. In chloride-Ringer, high [K+] increased the hydrosmotic responses to submaximal stimulations induced by vasopressin or exogenous cAMP, while the responses to theophylline or serosal hypertonicity were decreased. In sulfate-Ringer, all these responses were enhanced but for that induced by serosal hypertonicity which was actually diminished. As a step towards determining if Ca2+ might mediate the K+-induced effects on water flow, experiments were conducted either in the presence of a Ca2+ "antagonist" (cobalt) or in nominally Ca2+-free Ringer. In both conditions the hydrosmotic effects of vasopressin and cAMP were markedly reduced. The results raise the possibility that a transient Ca2+ influx through voltage-sensitive, Co2+-blockade Ca2+ channels may play a role in the stimulus-hydrosmotic response of toad urinary bladder.

Citing Articles

Mercury blockage of apical water channels in toad skin (Bufo marinus).

Grosso A, de Sousa R J Physiol. 1993; 468:741-52.

PMID: 8254534 PMC: 1143853. DOI: 10.1113/jphysiol.1993.sp019798.

Forskolin mimics the hydrosmotic action of vasopressin in the urinary bladder of toads Bufo marinus.

de Sousa R, Grosso A J Physiol. 1985; 365:307-18.

PMID: 2993597 PMC: 1193004. DOI: 10.1113/jphysiol.1985.sp015774.

Amiloride inhibits the vasopressin-induced increase in epithelial water permeability.

Grosso A, Cragoe Jr E, DESOUSA R Pflugers Arch. 1990; 417(2):200-6.

PMID: 1964723 DOI: 10.1007/BF00370700.

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