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Effect of Administration of the Carcinogen Dimethylnitrosamine on Urinary 7-methylguanine

Overview
Journal Biochem J
Specialty Biochemistry
Date 1967 Aug 1
PMID 6048785
Citations 12
Authors
V M Craddock
P N MAGEE
Affiliations
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Abstract

1. Evidence is presented for the excretion of 7-methylguanine in normal rat urine at a rate of approx. 65mug./day. Experiments with animals in which the nucleic acids had been prelabelled by treatment of the neonatal rats with [(14)C]-formate gave evidence that the methylated base originated in the nucleic acids of the rat. 2. Injection of [(14)C]dimethylnitrosamine leads to an increased excretion of 7-methylguanine, and the base becomes labelled in the methyl group. The disappearance of labelled 7-methylguanine formed in nucleic acids of rats treated with the carcinogen therefore does not take place by an N-demethylation reaction, but by liberation of the intact methylated base.

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Study of the methylation and lack of deamination of deoxyribonucleic acid by N-methyl-N'-nitro-N-nitrosoguanidine.

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Stability of deoxyribonucleic acid methylated in the intact animal by administration of dimethylnitrosamine. Rate of breakdown in vivo and in vitro at different dosages.

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References
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