Organ Specificity and Lactate-dehydrogenase Activity. Some Properties of Human Spermatozoal Lactate Dehydrogenase

Overview

Journal Biochem J

Specialty Biochemistry

Date 1965 Dec 1

PMID 5894044

Citations 12

Authors

J H Wilkinson

W A WITHYCOMBE

Affiliations

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Abstract

1. The presence of a characteristic lactate-dehydrogenase isoenzyme (LD(x)) in human, mouse and dog testis and in human spermatozoa has been confirmed by electrophoresis on cellulose acetate and on polyacrylamide gel. 2. The human spermatozoal isoenzyme exhibits a much higher affinity for 2-oxobutyrate than any of the five isoenzymes found in other tissues. K(m) values of 0.05mm for pyruvate and 0.18mm for 2-oxobutyrate were obtained. 3. LD(x) differs from other lactate-dehydrogenase isoenzymes in that its properties cannot be correlated with its electrophoretic mobility. It resembles LD(1) in being strongly inhibited by 0.2mm-oxalate and relatively resistant to 2m-urea, and in being relatively stable to heat. 4. The surprisingly high activity of LD(x) with 2-oxobutyrate suggests that this substance or 2-hydroxybutyrate may play a part in spermatozoal metabolism.

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References

Stadtman E . Symptosium on multiple forms of enzymes and control mechanisms. II. Enzyme multiplicity and function in the regulation of divergent metabolic pathways. Bacteriol Rev. 1963; 27:170-81. PMC: 441177. DOI: 10.1128/br.27.2.170-181.1963. View

Plummer D, Wilkinson J . Organ specificity and lactatedehydrogenase activity. 2. Some properties of human-heart and liver preparations. Biochem J. 1963; 87:423-9. PMC: 1201911. DOI: 10.1042/bj0870423. View

Rosalki S, Wilkinson J . Reduction of alpha-ketobutyrate by human serum. Nature. 1960; 188:1110-1. DOI: 10.1038/1881110a0. View

Yanofsky C . D-Serine dehydrase of Neurospora. J Biol Chem. 1952; 198(1):343-52. View

Wacker W, DORFMAN L . Urinary lactic dehydrogenase activity. I. Screening method for detection of cancer of kidneys and bladder. JAMA. 1962; 181:972-8. View

ZINKHAM W, Blanco A, KUPCHYK L . LACTATE DEHYDROGENASE IN TESTIS: DISSOCIATION AND RECOMBINATION OF SUBUNITS. Science. 1963; 142(3597):1303-4. DOI: 10.1126/science.142.3597.1303. View

Allen J . Multiple forms of lactic dehydrogenase in tissues of the mouse: their specificity, cellular localization, and response to altered physiological conditions. Ann N Y Acad Sci. 1961; 94:937-51. DOI: 10.1111/j.1749-6632.1961.tb35586.x. View

Plagemann P, Gregory K, WROBLEWSKI F . The electrophoretically distinct forms of mammalian lactic dehydrogenase. 1. Distribution of lactic dehydrogenase. 1. Distribution of lactic dehydrogenases in rabbit and human tissue. J Biol Chem. 1960; 235:2282-7. View

Appella E, MARKERT C . Dissociation of lactate dehydrogenase into subunits with guanidine hydrochloride. Biochem Biophys Res Commun. 1961; 6:171-6. DOI: 10.1016/0006-291x(61)90123-1. View

10.

Plummer D, Elliott B, COOKE K, Wilkinson J . Organ specificity and lactate-dehydrogenase activity. 1. The relative activities with pyruvate and 2-oxobutyrate of electrophoretically separated fractions. Biochem J. 1963; 87:416-22. PMC: 1201910. DOI: 10.1042/bj0870416. View

11.

Emerson P, Wilkinson J, WITHYCOMBE W . EFFECT OF OXALATE ON THE ACTIVITY OF LACTATE DEHYDROGENASE ISOENZYMES. Nature. 1964; 202:1337-8. DOI: 10.1038/2021337a0. View

12.

Yanofsky C, REISSIG J . L-Serine dehydrase of Neurospora. J Biol Chem. 1953; 202(2):567-77. View

13.

Raymond S, Weintraub L . Acrylamide gel as a supporting medium for zone electrophoresis. Science. 1959; 130(3377):711. DOI: 10.1126/science.130.3377.711. View

14.

Blanco A, ZINKHAM W . Lactate Dehydrogenases in Human Testes. Science. 1963; 139(3555):601-2. DOI: 10.1126/science.139.3555.601. View

15.

Raymond S . A convenient apparatus for vertical gel electrophoresis. Clin Chem. 1962; 8:455-70. View

16.

Menini E, NORYMBERSKI J . Determination of oxo steroids as coloured salts of their Girard derivatives. Biochem J. 1964; 93(1):11-4. PMC: 1206175. DOI: 10.1042/bj0930011. View

17.

MARKERT C . Lactate Dehydrogenase Isozymes: Dissociation and Recombination of Subunits. Science. 1963; 140(3573):1329-30. DOI: 10.1126/science.140.3573.1329. View

18.

Cahn R, ZWILLING E, Kaplan N, Levine L . Nature and Development of Lactic Dehydrogenases: The two major types of this enzyme form molecular hybrids which change in makeup during development. Science. 1962; 136(3520):962-9. DOI: 10.1126/science.136.3520.962. View

19.

WITHYCOMBE W, Plummer D, Wilkinson J . ORGAN SPECIFICITY AND LACTATE-DEHYDROGENASE ACTIVITY. DIFFERENTIAL INHIBITION BY UREA AND RELATED COMPOUNDS. Biochem J. 1965; 94:384-9. PMC: 1206520. DOI: 10.1042/bj0940384. View

20.

Goldberg E . Lactic and Malic Dehydrogenases in Human Spermatozoa. Science. 1963; 139(3555):602-3. DOI: 10.1126/science.139.3555.602. View