Isolation of Human Lactate Dehydrogenase Isoenzyme X by Affinity Chromatography

Overview

Journal Biochem J

Specialty Biochemistry

Date 1978 Sep 1

PMID 213050

Citations 3

Authors

A H Kolk

L van Kuyk

B Boettcher

Affiliations

Soon will be listed here.

Abstract

Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. In the presence of 1.6 mM-NAD+, isoenzyme LDH-X does not bind to AMP-Sepharose, whereas the other lactate dehydrogenase isoenzymes do. This is the crucial point in the isolation of isoenzyme LDH-X from the other isoenzymes. The purified human isoenzyme LDH-X had a specific activity of 146 units/mg of protein.

Citing Articles

A logical approach to the isolation of lactate dehydrogenase isozyme X from human testes: a general rationale for the isolation of homotetrameric LDH isozymes.

Toowicharanont P, Svasti J Experientia. 1980; 36(1):37-8.

PMID: 7358131 DOI: 10.1007/BF02003956.

Separation of rat lactate dehydrogenase isoenzyme C4 from other isoenzymes by affinity and ion-exchange chromatography.

Ansari A Biochem J. 1981; 199(1):75-9.

PMID: 7337716 PMC: 1163336. DOI: 10.1042/bj1990075.

Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli.

LeVan K, Goldberg E Biochem J. 1991; 273 ( Pt 3):587-92.

PMID: 1996957 PMC: 1149803. DOI: 10.1042/bj2730587.

References

Spielmann H, Erickson R, Epstein C . The separation of lactate dehydrogenase X from other lactate dehydrogenase isozymes of mouse testes by affinity chromatography. FEBS Lett. 1973; 35(1):19-23. DOI: 10.1016/0014-5793(73)80568-x. View

Wilkinson J, WITHYCOMBE W . Organ specificity and lactate-dehydrogenase activity. Some properties of human spermatozoal lactate dehydrogenase. Biochem J. 1965; 97(3):663-8. PMC: 1264743. DOI: 10.1042/bj0970663. View

OCARRA P, Barry S, Corcoran E . Affinity chromatographic differentiation of lactate dehydrogenase isoenzymes on the basis of differential abortive complex formation. FEBS Lett. 1974; 43(2):163-8. DOI: 10.1016/0014-5793(74)80992-0. View

Goldberg E, Lerum J . Pregnancy suppression by an antiserum to the sperm specific lactate dehydrogenase. Science. 1972; 176(4035):686-7. DOI: 10.1126/science.176.4035.686. View

OCARRA P, Barry S . Affinity chromatography of lactate dehydrogenase Model studies demonstrating the potential of the technique in the mechanistic investigation as well as in the purification of multi-substrate enzymes. FEBS Lett. 1972; 21(3):281-285. DOI: 10.1016/0014-5793(72)80183-2. View

Goldberg E . Lactate dehydrogenase-X from mouse testes and spermatozoa. Methods Enzymol. 1975; 41:318-23. DOI: 10.1016/s0076-6879(75)41072-2. View

Blanco A, ZINKHAM W . Lactate Dehydrogenases in Human Testes. Science. 1963; 139(3555):601-2. DOI: 10.1126/science.139.3555.601. View

Lerum J, Goldberg E . Immunological impairment of pregnancy in mice by lactate dehydrogenase-X. Biol Reprod. 1974; 11(1):108-15. DOI: 10.1095/biolreprod11.1.108. View

Goldberg E . Immunochemical specificity of lactate dehydrogenase-X. Proc Natl Acad Sci U S A. 1971; 68(2):349-52. PMC: 388935. DOI: 10.1073/pnas.68.2.349. View

10.

Dietz A, Lubrano T . Separation and quantitation of lactic dehydrogenase isoenzymes by disc electrophoresis. Anal Biochem. 1967; 20(2):246-57. DOI: 10.1016/0003-2697(67)90030-9. View

11.

MARKERT C . Lactate Dehydrogenase Isozymes: Dissociation and Recombination of Subunits. Science. 1963; 140(3573):1329-30. DOI: 10.1126/science.140.3573.1329. View

12.

Mosbach K, Guilford H, Ohlsson R, Scott M . General ligands in affinity chromatography. Cofactor-substrate elution of enzymes bound to the immobilized nucleotides adenosine 5'-monophosphate and nicotinamide-adenine dinucleotide. Biochem J. 1972; 127(4):625-31. PMC: 1178759. DOI: 10.1042/bj1270625. View

13.

Goldberg E . Infertility in female rabbits immunized with lactate dehydrogenase X. Science. 1973; 181(4098):458-9. DOI: 10.1126/science.181.4098.458. View

14.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

15.

Goldberg E . Lactic and Malic Dehydrogenases in Human Spermatozoa. Science. 1963; 139(3555):602-3. DOI: 10.1126/science.139.3555.602. View

16.

ZINKHAM W, Blanco A, KUPCHYK L . LACTATE DEHYDROGENASE IN PIGEON TESTES: GENETIC CONTROL BY THREE LOCI. Science. 1964; 144(3624):1353-4. DOI: 10.1126/science.144.3624.1353. View

17.

Goldberg E, Hawtrey C . The ontogeny of sperm specific lactate dehydrogenase in mice. J Exp Zool. 1967; 164(3):309-16. DOI: 10.1002/jez.1401640302. View

18.

Goldberg E . Amino acid composition and properties of crystalline lactate dehydrogenase X from mouse testes. J Biol Chem. 1972; 247(7):2044-8. View

19.

Erickson R, Hoppe P, Tennenbaum D, Spielmann H, Epstein C . Lactate dehydrogenase X: effects of antibody on mouse gametes but not on early development. Science. 1975; 188(4185):261-3. DOI: 10.1126/science.1167979. View