Primary Alcohol Sulfatase in a Pseudomonas Species

Overview

Journal Appl Microbiol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1965 Sep 1

PMID 5867650

Citations 14

Authors

W J Payne

J P Williams

W R Mayberry

Affiliations

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Abstract

An ammonium sulfate-precipitated fraction from cell-free extracts of Pseudomonas C12B grown on a medium containing sodium dodecyl sulfate (SDS) contained alkyl sulfatase increased fourfold in specific activity over the crude. Optimal pH (7.5) and temperature (70 C) for sulfate release were determined with SDS labeled with radioactive sulfur (SDS(35)) as test substrate. Phosphate, arsenate, and certain heavy metal ions inhibited desulfation, whereas Mg(++) and Mn(++) stimulated activity of preparations which had been dialyzed against ethylenediaminetetraacetic acid. Dodecanol was recovered in semiquantitative yield from reaction mixtures containing enzyme and SDS(35). Aryl sulfates, secondary alcohol sulfates, and a phenoxyethyl sulfate failed to serve as substrate for this enzyme.

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Further studies on the substrate specificity and inhibition of the stereospecific CS2 secondary alkylsulphohydrolase of Comamonas terrigena.

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Purification and properties of the P2 primary alkylsulphohydrolase of the detergent-degrading bacterium pseudomonas C12B.

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References

DODGSON K, MELVILLE T, Spencer B, Williams K . Studies on sulphatases. VIII. The arylsulphatase of a strain of Alcaligenes metalcaligenes isolated from intertidal mud. Biochem J. 1954; 58(2):182-7. PMC: 1269868. DOI: 10.1042/bj0580182. View

CAMPBELL Jr L . Purification and properties of an alpha-amylase from facultative thermophilic bacteria. Arch Biochem Biophys. 1955; 54(1):154-61. DOI: 10.1016/0003-9861(55)90018-7. View

Payne W, Feisal V . Bacterial utilization of dodecyl sulfate and dodecyl benzene sulfonate. Appl Microbiol. 1963; 11:339-44. PMC: 1057999. DOI: 10.1128/am.11.4.339-344.1963. View

Hsu Y . DETERGENT (SODIUM LAURYL SULPHATE)-SPLITTING ENZYME FROM BACTERIA. Nature. 1963; 200:1091-2. DOI: 10.1038/2001091b0. View

FOWLER L, RAMMLER D . SULFUR METABOLISM OF AEROBACTOR AEROGENES. II. THE PURIFICATION AND SOME PROPERTIES OF A SULFATASE. Biochemistry. 1964; 3:230-7. DOI: 10.1021/bi00890a015. View