Induction of Primary Alkysulphatases and Metabolism of Sodium Hexan-1-yl Sulphate by Pseudomonas C12B

Overview

Journal Biochem J

Specialty Biochemistry

Date 1974 Jan 1

PMID 4840838

Citations 4

Authors

J W Fitzgerald

K S DODGSON

W J Payne

Affiliations

Soon will be listed here.

Abstract

Sodium hexan-1-yl sulphate and certain related alkyl sulphate esters have been shown to serve as inducers of the formation of primary alkylsulphatases (designated as P1 and P2) in Pseudomonas C12B. When the organism is grown on sodium hexan-1-yl [(35)S]sulphate as the sole source of sulphur or as the sole source of carbon and sulphur only the P2 alkylsulphatase is formed and inorganic (35)SO(4) (2-) is liberated into the media. Cell extracts contain this anion as the major (35)S-labelled metabolite although two unidentified labelled metabolites as well as choline O-[(35)S]sulphate occur in trace quantities in some extracts. Dialysed cell extracts are capable of liberating inorganic (35)SO(4) (2-) from sodium hexan-1-yl [(35)S]sulphate without the need to include cofactors known to be required for the bacterial degradation of n-alkanes. The collective results suggest that sodium hexan-1-yl sulphate can act as an inducer of P1 alkylsulphatase formation without the need for prior metabolic modification of the carbon moiety of the ester.

Citing Articles

Specificity of P2 primary alkylsulphohydrolase induction in the detergent-degrading bacterium Pseudomonas C12B. Effects of alkanesulphonates, alkyl sulphates and other related compounds.

Cloves J, DODGSON K, White G, Fitzgerald J Biochem J. 1980; 185(1):13-21.

PMID: 6246873 PMC: 1161264. DOI: 10.1042/bj1850013.

Chemically defined inducers of alkylsulphatases present in Pseudomonas C12B.

DODGSON K, Fitzgerald J, Payne W Biochem J. 1974; 138(1):53-62.

PMID: 4840837 PMC: 1166174. DOI: 10.1042/bj1380053.

Secondary alkylsulphatases in a strain of Comamonas terrigena.

Fitzgerald J Biochem J. 1975; 149(2):477-80.

PMID: 1180908 PMC: 1165642. DOI: 10.1042/bj1490477.

Sulfate ester formation and hydrolysis: a potentially important yet often ignored aspect of the sulfur cycle of aerobic soils.

Fitzgerald J Bacteriol Rev. 1976; 40(3):698-721.

PMID: 791238 PMC: 413977. DOI: 10.1128/br.40.3.698-721.1976.

References

Payne W, Williams J, Mayberry W . Primary alcohol sulfatase in a Pseudomonas species. Appl Microbiol. 1965; 13(5):698-701. PMC: 1058327. DOI: 10.1128/am.13.5.698-701.1965. View

Scott J, Spencer B . Regulation of choline sulphatase synthesis and activity in Aspergillus nidulans. Biochem J. 1968; 106(2):471-7. PMC: 1198525. DOI: 10.1042/bj1060471. View

Lloyd A, Large P, Davies M, OLAVESEN A, DODGSON K . The glycosulphatase of Trichoderma viride. Biochem J. 1968; 108(3):393-9. PMC: 1198824. DOI: 10.1042/bj1080393. View

Denner W, OLAVESEN A, Powell G, DODGSON K . The metabolism of potassium dodecyl [35-S]sulphate in the rat. Biochem J. 1969; 111(1):43-51. PMC: 1187492. DOI: 10.1042/bj1110043. View

Phibbs Jr P, Eagon R . Transport and phosphorylation of glucose, fructose, and mannitol by Pseudomonas aeruginosa. Arch Biochem Biophys. 1970; 138(2):470-82. DOI: 10.1016/0003-9861(70)90371-1. View

Ottery J, OLAVESEN A, DODGSON K . Metabolism of dodecyl sulphate in the rat: non-enzymic liberation of sulphate and gamma-butyroactone from the major metabolite, butyric acid 4-sulphate. Life Sci II. 1970; 9(23):1335-40. DOI: 10.1016/0024-3205(70)90114-1. View

Fitzgerald J, DODGSON K . Carbon and sulphur utilization during growth of Pseudomonas fluorescens on potassium D-glucose 6-O-sulphate as the sole sulphur source. Biochem J. 1971; 122(3):277-83. PMC: 1176776. DOI: 10.1042/bj1220277. View

Fitzgerald J, DODGSON K . Sulphur utilization during growth of pseudomonas fluorescens on potassium D-glucose 6-O-sulphate. Biochem J. 1971; 121(3):521-8. PMC: 1176600. DOI: 10.1042/bj1210521. View

Lucas J, Burchiel S, Segel I . Choline sulfatase of Pseudomonas aeruginosa. Arch Biochem Biophys. 1972; 153(2):664-72. DOI: 10.1016/0003-9861(72)90385-2. View

10.

Klug M, Markovetz A . Utilization of aliphatic hydrocarbons by micro-organisms. Adv Microb Physiol. 1971; 5:1-43. DOI: 10.1016/s0065-2911(08)60404-x. View

11.

Payne W, PAINTER B . Resolution by acrylamide gel electrophoresis of alkyl sulphatases and alcohol dehydrogenase. Microbios. 1971; 3(12):199-206. View

12.

Fitzgerald J . The formation of choline O-sulphate by Pseudomonas C12B and other Pseudomonas species. Biochem J. 1973; 136(2):361-9. PMC: 1165962. DOI: 10.1042/bj1360361. View

13.

Fitzgerald J, Payne W . Induction in a Pseudomonas species of sulphatases active on short chain alkylsulphates. Microbios. 1972; 5(18):87-100. View

14.

DODGSON K, Fitzgerald J, Payne W . Chemically defined inducers of alkylsulphatases present in Pseudomonas C12B. Biochem J. 1974; 138(1):53-62. PMC: 1166174. DOI: 10.1042/bj1380053. View

15.

DODGSON K, Lloyd A, Tudball N . O-sulphate esters of L-serine, L-threonine and L-hydroxyproline. Biochem J. 1961; 79:111-7. PMC: 1205555. DOI: 10.1042/bj0790111. View

16.

DODGSON K . Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate esters. Biochem J. 1961; 78:312-9. PMC: 1205268. DOI: 10.1042/bj0780312. View

17.

Lloyd A, Tudball N, DODGSON K . Infrared studies on sulphate esters. III. O-Sulphate esters of alcohols, amino alcohols and hydroxylated amino acids. Biochim Biophys Acta. 1961; 52:413-9. DOI: 10.1016/0006-3002(61)90397-3. View

18.

Takebe I . Isolation and characterization of a new enzyme choline sulfatase. J Biochem. 1961; 50:245-55. DOI: 10.1093/oxfordjournals.jbchem.a127440. View