The Linkage Between the Polysaccharide and Mucopeptide Components of the Cell Wall of Lactobacillus Casei

Overview

Journal Biochem J

Specialty Biochemistry

Date 1965 Aug 1

PMID 5837777

Citations 18

Authors

K W KNOX

E A Hall

Affiliations

Soon will be listed here.

Abstract

1. The linkage between the polysaccharide and mucopeptide components of the cell wall of Lactobacillus casei is rapidly hydrolysed under mild acid-hydrolysis conditions. 2. The release of the polysaccharide is accompanied by the hydrolysis of an N-acetylhexosaminide linkage. The N-acetylhexosamine residue readily forms chromogen and it is concluded that it is substituted on C((3)) by the adjacent sugar. 3. Continued heating of the polysaccharide in acid results in a slower release of reactive N-acetylhexosamine due to the hydrolysis of glycosidic linkages within the polysaccharide. 4. After the linkage between the polysaccharide and mucopeptide has been hydrolysed, acid phosphatase will release approx. 40% of the total phosphorus as inorganic phosphate. 5. It is concluded that the polysaccharide component of the cell wall is joined through its reducing end group to a phosphate grouping in the mucopeptide.

Citing Articles

Teichoic and teichuronic acids: biosynthesis, assembly, and location.

Ward J Microbiol Rev. 1981; 45(2):211-43.

PMID: 7022154 PMC: 281507. DOI: 10.1128/mr.45.2.211-243.1981.

The teichuronic acid from the walls of Bacillus licheniformis A.T.C.C. 9945.

Lifely M, Tarelli E, BADDILEY J Biochem J. 1980; 191(2):305-18.

PMID: 6263243 PMC: 1162220. DOI: 10.1042/bj1910305.

The linkage between teichoic acid and glycosaminopeptide in the walls of a strain of Staphylococcus lactis.

Button D, Archibald A, BADDILEY J Biochem J. 1966; 99(2):11C-14C.

PMID: 5947143 PMC: 1265022. DOI: 10.1042/bj0990011c.

Properties of the polysaccharide and mucopeptide components of the cell wall of Lactobacillus casei.

Hall E, KNOX K Biochem J. 1965; 96(2):310-8.

PMID: 5837778 PMC: 1207041. DOI: 10.1042/bj0960310.

The glycerol teichoic acid of walls of Staphylococcus lactis I3.

Archibald A, BADDILEY J, Button D Biochem J. 1968; 110(3):543-57.

PMID: 5701685 PMC: 1187384. DOI: 10.1042/bj1100543.

References

Kelemen M, BADDILEY J . Structure of the intracellular glycerol teichoic acid from Lactobacillus casei A.T.C.C. 7469. Biochem J. 1961; 80:246-54. PMC: 1243990. DOI: 10.1042/bj0800246. View

KNOX K, BRANDSEN J . The isolation of components from the cell wall of Lactobacillus casei. Biochem J. 1962; 85:15-23. PMC: 1243905. DOI: 10.1042/bj0850015. View

DISCHE Z . Qualitative and quantitative colorimetric determination of heptoses. J Biol Chem. 1953; 204(2):983-97. View

Glomset J . The further purification and properties of a phosphatase from spleen able to hydrolyze completely the phosphorus of alpha-casein. Biochim Biophys Acta. 1959; 32:349-57. DOI: 10.1016/0006-3002(59)90606-7. View

Strominger J, Ghuysen J . ON THE LINKAGE BETWEEN TEICHOIC ACID AND THE GLYCOPEPTIDE IN THE CELL WALL OF STAPHYLOCOCCUS AUREUS. Biochem Biophys Res Commun. 1963; 12:418-24. DOI: 10.1016/0006-291x(63)90117-7. View

Aminoff D, MORGAN W, WATKINS W . Studies in immunochemistry. 11. The action of dilute alkali on the N-acetylhexosamines and the specific blood-group mucoids. Biochem J. 1952; 51(3):379-89. PMC: 1197857. DOI: 10.1042/bj0510379. View

SHOCKMAN G, Kolb J, TOENNIES G . A high speed shaker for the disruption of cells at low temperatures. Biochim Biophys Acta. 1957; 24(1):203-4. DOI: 10.1016/0006-3002(57)90168-3. View

Ikawa M . The partial chemical degradation of the cell walls of Lactobacillus plantarum, Streptococcus faecalis, and Lactobacillus casei. J Biol Chem. 1961; 236:1087-92. View

Ellwood D, Kelemen M, BADDILEY J . The glycerol teichoic acid from the walls of Staphylococcus albus N.T.C.C. 7944. Biochem J. 1963; 86(2):213-25. PMC: 1201738. DOI: 10.1042/bj0860213. View

10.

Krause R, McCarty M . Studies on the chemical structure of the streptococcal cell wall. I. The identification of a mucopeptide in the cell walls of groups A and A-variant streptococci. J Exp Med. 1961; 114:127-40. PMC: 2137441. DOI: 10.1084/jem.114.1.127. View

11.

Perkins H . Chemical structure and biosynthesis of bacterial cell walls. Bacteriol Rev. 1963; 27:18-55. PMC: 441168. DOI: 10.1128/br.27.1.18-55.1963. View

12.

Hofman T . Studies on casein. 1. Purification and properties of phosphoprotein phosphatase. Biochem J. 1958; 69(1):135-9. PMC: 1196525. DOI: 10.1042/bj0690135. View

13.

Krause R, McCarty M . Studies on the chemical structure of the streptococcal cell wall. II. The composition of group C cell walls and chemical basis for serologic specificity of the carbohydrate moiety. J Exp Med. 1962; 115:49-62. PMC: 2137474. DOI: 10.1084/jem.115.1.49. View

14.

Huggett A, Nixon D . Use of glucose oxidase, peroxidase, and O-dianisidine in determination of blood and urinary glucose. Lancet. 1957; 273(6991):368-70. DOI: 10.1016/s0140-6736(57)92595-3. View

15.

KNOX K, Hall E . THE ISOLATION OF OLIGOSACCHARIDES FROM THE CELL-WALL POLYSACCHARIDE OF LACTOBACILLUS CASEI, SEROLOGICAL GROUP C. Biochem J. 1965; 94:525-33. PMC: 1206584. DOI: 10.1042/bj0940525. View

16.

Hall E, KNOX K . Properties of the polysaccharide and mucopeptide components of the cell wall of Lactobacillus casei. Biochem J. 1965; 96(2):310-8. PMC: 1207041. DOI: 10.1042/bj0960310. View

17.

Sharpe M, WHEATER D . Lactobacillus helveticus. J Gen Microbiol. 1957; 16(3):676-9. DOI: 10.1099/00221287-16-3-676. View

18.

Sharpe M . A serological classification of lactobacilli. J Gen Microbiol. 1955; 12(1):107-22. DOI: 10.1099/00221287-12-1-107. View

19.

Cessi C, SERAFINI-CESSI F . A method for the determination of D-galactosamine in the presence of D-glucosamine. Biochem J. 1963; 88:132-6. PMC: 1203861. DOI: 10.1042/bj0880132. View

20.

Archibald A, Armstrong J, BADDILEY J, Hay J . Teichoic acids and the structure of bacterial walls. Nature. 1961; 191:570-2. DOI: 10.1038/191570a0. View