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Radiation-induced Breaks of DNA in Cultured Mammalian Cells

Overview
Journal Biophys J
Publisher Cell Press
Specialty Biophysics
Date 1969 Mar 1
PMID 5780712
Citations 27
Authors
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Abstract

Mouse leukemic cells (L5178Y) in suspension culture were irradiated and the extent of single-strand breaks and double-strand cuts of DNA was estimated by sucrose gradient centrifugation. The radiation produced 3.0 single-strand breaks per cell (G(1) stage) per rad and approximately 0.3 double-strand breaks per cell (G(1) stage) per rad.

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References
1.
Okada S . Replicating units (replicons) of DNA in cultured mammalian cells. Biophys J. 1968; 8(5):650-64. PMC: 1367405. DOI: 10.1016/S0006-3495(68)86513-0. View

2.
Boyce R, Tepper M . X-ray-induced single-strand breaks and joining of broken strands in superinfecting lambda DNA in Escherichia coli lysogenic for lambda. Virology. 1968; 34(2):344-51. DOI: 10.1016/0042-6822(68)90245-6. View

3.
Cole A, HUMPHREY R, Dewey W . LOW-VOLTAGE ELECTRON BEAM IRRADIATION OF NORMAL AND 5-BROMOURIDINE DEOXYRIBOSIDE-TREATED L-P59 MOUSE FIBROBLAST CELLS IN VITRO. Nature. 1963; 199:780-2. DOI: 10.1038/199780a0. View

4.
Burgi E, HERSHEY A . Sedimentation rate as a measure of molecular weight of DNA. Biophys J. 1963; 3:309-21. PMC: 1366449. DOI: 10.1016/s0006-3495(63)86823-x. View

5.
Englander S, Buzzell A, LAUFFER M . The relationship between inactivation of tobacco mosaic virus by x-rays and breakage of nucleic acid. Biochim Biophys Acta. 1960; 40:385-92. DOI: 10.1016/0006-3002(60)91378-0. View