Effective Specific Activity Dilution in Labeled DNA of Cultured Mammalian Cells (L5178Y)

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1969 Feb 1

PMID 5764222

Citations 1

Authors

H J Burki

S Okada

Affiliations

Soon will be listed here.

Abstract

Upon labeling the DNA of cultured mammalian cells with radio-active thymidine it was found that there was a large reduction in the specific activity of radioactive thymidine in DNA when this specific activity was compared to the specific activity of the exogenous supply. This reduction in specific activity may be determined once the effective dilution factor for thymidine in mammalian cells is known. The average effective dilution factor was found to be 2 x 10(-4) M for L5178Y cells in log growth.

Citing Articles

Radiation-induced breaks of DNA in cultured mammalian cells.

Veatch W, Okada S Biophys J. 1969; 9(3):330-46.

PMID: 5780712 PMC: 1367572. DOI: 10.1016/S0006-3495(69)86390-3.

References

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