Evidence for Histidine in the Triethyltin-binding Site of Rat Haemoglobin

Overview

Journal Biochem J

Specialty Biochemistry

Date 1969 Jan 1

PMID 5763785

Citations 18

Authors

M S Rose

Affiliations

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Abstract

One molecule of rat haemoglobin binds two molecules of triethyltin. The binding sites are located on the globin and there is co-operativity between the sites such that the intrinsic affinity constant at pH8.0 increases from 3.5x10(5)m(-1) for the binding of the first triethyltin molecule to 5.0x10(5)m(-1) for the binding of the second. Evidence is presented, from pH studies and the kinetics of inhibition due to photo-oxidation, that each binding site contains two histidine residues.

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The interaction of triethyltin with a component of guinea-pig liver supernatant. Evidence for histidine in the binding sites.

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