Mutants of Sindbis Virus. III. Host Polypeptides Present in Purified HR and Ts103 Virus Particles

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1978 Nov 1

PMID 569218

Citations 14

Authors

E G Strauss

Affiliations

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Abstract

The amounts of host-encoded protein present in purified Sindbis virions of both the HR strain and of a mutant (ts103) which makes multicored particles were examined. Cells were labeled with [35S]methionine before infection and with [3H]methionine postinfection. Virions were purified by velocity sedimentation and isopycnic banding, and their polypeptides were examined by polyacrylamide gels in a sodium dodecyl sulfate-containing discontinuous buffer system. Host prelabeled material was found principally in a small number of discrete polypeptides in HR virions, which contained as little as 0.2% host-encoded protein. Virus-sized particles of mutant ts103 contained significantly more host material, and multiploid particles from ts103 infection contained up to 12% host prelabeled protein.

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