DNA Replication in Physarum Polycephalum: Bidirectional Replication of DNA Within Replicons

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1978 Mar 1

PMID 565510

Citations 6

Authors

S Funderud

R Andreassen

F Haugli

Affiliations

Soon will be listed here.

Abstract

The direction of replication of DNA within replicons of Physarum polycephalum was studied by pulse-labelling with 5-bromouracil-deoxyriboside (BrdUrd) and 3H-adenosine deoxyriboside (dAdo), followed by ultraviolet- (UV) -photolysis and analysis of molecular weights of single strand DNA fragments on alkaline sucrose gradients. Newly made DNA within replicons at all stages of completion is split in two equal halves upon UV irradiation when BrdUrd was given at the time of initiation of DNA synthesis. This shows that replication within replicons of Physarum polycephalum starts at an origin located in the center of each unit, proceeding bidirectionally from this origin.

Citing Articles

Replication initiation and elongation fork rates within a differentially expressed human multicopy locus in early S phase.

Scott R, Truong K, Vos J Nucleic Acids Res. 1998; 25(22):4505-12.

PMID: 9358159 PMC: 147096. DOI: 10.1093/nar/25.22.4505.

Mapping of a replication origin within the promoter region of two unlinked, abundantly transcribed actin genes of Physarum polycephalum.

Benard M, Lagnel C, Pallotta D, Pierron G Mol Cell Biol. 1996; 16(3):968-76.

PMID: 8622700 PMC: 231079. DOI: 10.1128/MCB.16.3.968.

Two alleles of a developmentally regulated alpha-tubulin locus in Physarum polycephalum replicate on different schedules.

Cunningham D, Dove W Mol Cell Biol. 1993; 13(1):449-61.

PMID: 8093328 PMC: 358925. DOI: 10.1128/mcb.13.1.449-461.1993.

Isolation of newly replicated chromatin by using shallow metrizamide gradients.

Murphy R, Wallace R, Bonner J Proc Natl Acad Sci U S A. 1980; 77(6):3336-40.

PMID: 6932022 PMC: 349610. DOI: 10.1073/pnas.77.6.3336.

DNA replication in Physarum polycephalum: electron microscopic and autoradiographic analysis of replicating DNA from defined stages of the S-period.

Funderud S, Andreassen R, Haugli F Nucleic Acids Res. 1979; 6(4):1417-31.

PMID: 450701 PMC: 327780. DOI: 10.1093/nar/6.4.1417.

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