Ferric Chloride Spray Detector for Cholesterol and Cholesteryl Esters on Thin-layer Chromatograms

Overview

Journal J Lipid Res

Publisher Elsevier

Specialty Biochemistry

Date 1968 May 1

PMID 5646191

Citations 22

Authors

R R Lowry

Affiliations

Soon will be listed here.

Abstract

The use of a stable solution of ferric chloride for the detection of cholesterol and cholesteryl esters on thin-layer chromatographic layers is described. Its advantages over the commonly used Carr-Price reagent are listed, and the levels of sensitivity for the new reagent are shown. Detection of other lipid classes is also possible.

Citing Articles

Single-molecule localization microscopy reveals STING clustering at the trans-Golgi network through palmitoylation-dependent accumulation of cholesterol.

Kemmoku H, Takahashi K, Mukai K, Mori T, Hirosawa K, Kiku F Nat Commun. 2024; 15(1):220.

PMID: 38212328 PMC: 10784591. DOI: 10.1038/s41467-023-44317-5.

HIV-1 Gag targeting to the plasma membrane reorganizes sphingomyelin-rich and cholesterol-rich lipid domains.

Tomishige N, Bin Nasim M, Murate M, Pollet B, Didier P, Godet J Nat Commun. 2023; 14(1):7353.

PMID: 37990014 PMC: 10663554. DOI: 10.1038/s41467-023-42994-w.

A novel sterol-binding protein reveals heterogeneous cholesterol distribution in neurite outgrowth and in late endosomes/lysosomes.

Yamaji-Hasegawa A, Murate M, Inaba T, Dohmae N, Sato M, Fujimori F Cell Mol Life Sci. 2022; 79(6):324.

PMID: 35644822 PMC: 11072113. DOI: 10.1007/s00018-022-04339-6.

Extracellular Vesicles Regulate Biofilm Formation and Yeast-to-Hypha Differentiation in Candida albicans.

Honorato L, de Araujo J, Ellis C, Piffer A, Pereira Y, Frases S mBio. 2022; 13(3):e0030122.

PMID: 35420476 PMC: 9239257. DOI: 10.1128/mbio.00301-22.

Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membrane.

Kishimoto T, Mioka T, Itoh E, Williams D, Andersen R, Tanaka K Mol Biol Cell. 2021; 32(15):1374-1392.

PMID: 34038161 PMC: 8694040. DOI: 10.1091/mbc.E20-11-0699.