The Effect of Chemical Treatments of Albumin and Orosomucoid on Rate of Clearance from the Rat Bloodstream and Rate of Pinocytic Capture of Rat Yolk Sac Cultured in Vitro

Overview

Journal Biochem J

Specialty Biochemistry

Date 1977 Jun 15

PMID 560846

Citations 23

Authors

A T Moore

K E Williams

J B Lloyd

Affiliations

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Abstract

Portions of a 125I-iodinated bovine serum albumin preparation were exposed to freezing, acetic acid (pH 3.5, 3.0 or 2.5), urea or formaldehyde, and the effect of these treatments on the rates of pinocytic uptake by yolk sacs from 17.5-day-pregnant rats cultured in vitro and of clearance from the rat bloodstream were studied. Uptake of albumin by the yolk sac was followed by rapid release of [125I]iodo-L-tyrosine into the culture medium. Similarly clearance of albumin in vivo was accompanied by the appearance of trichloroacetic acid-soluble radioactivity in the bloodstream. In both systems the rates of uptake of modified albumin preparations formed a series: formaldehyde or urea greater than acetic acid greater than freezing. The increased rates of uptake of modified albumin preparations could not be ascribed to the formation of aggregates nor, in the yolk-sac system, to an increase in the rate of pinosome formation. It is concluded that the various treatments to which the albumin was subjected increase to varying degrees the affinity of the albumin molecule for binding sites on that region of the plasma membrane from which pinocytic vesicles are formed. Some comparable experiments with native and desialylated human orosomucoid indicate that the rat yolk-sac epithelial cells do not possess the recognition system for uptake of asialoglycoproteins that exists on the surface of hepatic parenchymal cells.

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References

Andrews P . Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964; 91(2):222-33. PMC: 1202876. DOI: 10.1042/bj0910222. View

Bocci V . Metabolism of plasma proteins. Arch Fisiol. 1970; 67(4):314-444. View

ASHWELL G, Morell A . The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins. Adv Enzymol Relat Areas Mol Biol. 1974; 41(0):99-128. DOI: 10.1002/9780470122860.ch3. View

Labadie J, Chapman K, ARONSON Jr N . Glycoprotein catabolism in rat liver: Lysosomal digestion of iodinated asialo-fetuin. Biochem J. 1975; 152(2):271-9. PMC: 1172469. DOI: 10.1042/bj1520271. View

Williams K, Kidston E, Beck F, Lloyd J . Quantitative studies of pinocytosis. II. Kinetics of protein uptake and digestion by rat yolk sac cultured in vitro. J Cell Biol. 1975; 64(1):123-34. PMC: 2109475. DOI: 10.1083/jcb.64.1.123. View

Normann S . Kinetics of phagocytosis. 3. Two colloid reactions, competitive inhibition, and degree of inhibition between similar and dissimilar foreign particles. Lab Invest. 1974; 31(3):286-93. View

Normann S . Kinetics of phagocytosis. II. Analysis of in vivo clearance with demonstration of competitive inhibition between similar and dissimilar foreign particles. Lab Invest. 1974; 31(2):161-9. View

Sherman L, Harwig S, Hayne O . Macromolecular complexes formed as the result of chloramine-T radioiodination of proteins. Int J Appl Radiat Isot. 1974; 25(2):81-5. DOI: 10.1016/0020-708x(74)90103-3. View

Normann S . The kinetics of phagocytosis. 1. A study on the clearance of denatured bovine albumin and its competitive inhibition by denatured human albumin. J Reticuloendothel Soc. 1973; 14(6):587-98. View

10.

Buys C, Elferink M, BOUMA J, Gruber M, Nieuwenhuis P . Proteolysis of formaldehyde-treated albumin in Kupffer cells and its inhibition by suramin. J Reticuloendothel Soc. 1973; 14(2):209-23. View

11.

Williams K, Kidston E, Beck F, Lloyd J . Quantitative studies of pinocytosis. I. Kinetics of uptake of (125I)polyvinylpyrrolidone by rat yolk sac cultured in vitro. J Cell Biol. 1975; 64(1):113-22. PMC: 2109486. DOI: 10.1083/jcb.64.1.113. View

12.

Buys C, Dejong A, BOUMA J, Gruber M . Rapid uptake by liver sinusoidal cells of serum albumin modified with retention of its compact conformation. Biochim Biophys Acta. 1975; 392(1):95-100. DOI: 10.1016/0304-4165(75)90169-5. View

13.

Williams K, Lloyd J, Davies M, Beck F . Digestion of an exogenous protein by rat yolk-sac cultured in vitro. Biochem J. 1971; 125(1):303-8. PMC: 1178054. DOI: 10.1042/bj1250303. View

14.

Morell A, Gregoriadis G, Scheinberg I, Hickman J, ASHWELL G . The role of sialic acid in determining the survival of glycoproteins in the circulation. J Biol Chem. 1971; 246(5):1461-7. View

15.

Gregoriadis G, Morell A, Sternlieb I, Scheinberg I . Catabolism of desialylated ceruloplasmin in the liver. J Biol Chem. 1970; 245(21):5833-7. View

16.

Bertini F, Mego J, McQUEEN J . Distribution in tissue homogenates, and the nature of the linkage of injected proteins to subcellular particles. J Cell Physiol. 1967; 70(1):105-14. DOI: 10.1002/jcp.1040700114. View

17.

Poortmans J . [Electrophoretic and immuno-electrophoretic analyses of heat-soluble proteins]. Clin Chim Acta. 1962; 7:334-45. DOI: 10.1016/0009-8981(62)90032-3. View

18.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

19.

Roberts A, Nicholls S, Griffiths P, Williams K, Lloyd J . A quantitative study of pinocytosis and lysosome function in experimentally induced lysosomal storage. Biochem J. 1976; 160(3):621-9. PMC: 1164279. DOI: 10.1042/bj1600621. View

20.

MOXON L, WILD A . Localisation of proteins in coated micropinocytotic vesicles during transport across rabbit yolk sac endoderm. Cell Tissue Res. 1976; 171(2):175-93. DOI: 10.1007/BF00219405. View