R Factor Deoxyribonucleic Acid in Chromosomeless Progeny of Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1971 Feb 1

PMID 5541537

Citations 14

Authors

J Inselburg

Affiliations

Soon will be listed here.

Abstract

The deoxyribonucleic acid (DNA) of resistance (R) factor 222 carried by Escherichia coli strain P678-54 was found in the normally chromosomeless progeny (minicells) of that strain. The entry of the R222 DNA into minicells appears to be via segregation at the time of their formation from normal cells. The R222 DNA can replicate in minicells although the extent of its replication appears to be limited. An analysis of the R222 DNA structure indicates that it exists in minicells as double-stranded linear, open circular, and twisted circular monomers (molecular weight, about 6.2 x 10(7) daltons). The monomers visualized by electron microscopy are 31.0 +/- 0.5 mum in length. An examination of the effect of acridine orange on the replication of R222 and colicin E1 DNA indicates the dye intereferes with plasmid DNA replication.

Citing Articles

Visualization of membrane-associated R-plasmid DNA in fraction of Escherichia coli minicell lyzate.

Hochmannova J, Nesvera J, Stokrova J Folia Microbiol (Praha). 1980; 25(1):11-5.

PMID: 6986329 DOI: 10.1007/BF02876392.

Isolation of catenated and replicating DNA molecules of colicin factor E1 from minicells.

Inselburg J, Fuke M Proc Natl Acad Sci U S A. 1971; 68(11):2839-42.

PMID: 4941988 PMC: 389538. DOI: 10.1073/pnas.68.11.2839.

Physical and functional characteristics of R-factor deoxyribonucleic acid segregated into Escherichia coli minicells.

Levy S J Bacteriol. 1971; 108(1):300-8.

PMID: 4941560 PMC: 247066. DOI: 10.1128/jb.108.1.300-308.1971.

R factor proteins synthesized in Escherichia coli minicells: membrane-associated R factor proteins.

Levy S, McMurry L, Palmer E J Bacteriol. 1974; 120(3):1464-71.

PMID: 4612024 PMC: 245934. DOI: 10.1128/jb.120.3.1464-1471.1974.

R factor proteins synthesized in Escherichia coli minicells: incorporation studies with different R factors and detection of deoxyribonucleic acid-binding proteins.

Levy S J Bacteriol. 1974; 120(3):1451-63.

PMID: 4612023 PMC: 245933. DOI: 10.1128/jb.120.3.1451-1463.1974.

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