Physical and Functional Characteristics of R-factor Deoxyribonucleic Acid Segregated into Escherichia Coli Minicells

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1971 Oct 1

PMID 4941560

Citations 24

Authors

S B Levy

Affiliations

Soon will be listed here.

Abstract

Both fi(+) and fi(-) type R factors function as intact replicative units when segregated into Escherichia coli minicells. Hybridization studies demonstrate that at least 95% of the deoxyribonucleic acid (DNA) in R(+) minicells is episomal in origin. About half of the DNA can be extracted in a closed circular form and about 75% of the DNA is membrane associated. DNA, ribonucleic acid, and protein synthesis proceeds in R(+) minicells in contrast to R(-) minicell controls. The system offers a unique opportunity to study a relatively small replicative unit in a native cell environment and a simple means of isolating large quantities of episomal DNA.

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Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments.

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Biochemical and immunological characterization of an R plasmid-encoded protein with properties resembling those of major cellular outer membrane proteins.

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