Strandedness of Vicia Faba Chromosomes As Revealed by Enzyme Digestion Studies

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1965 Jul 1

PMID 5323605

Citations 20

Authors

J E Trosko

S Wolff

Affiliations

Soon will be listed here.

Abstract

Chromosomes and nuclei isolated from neutral formalin-fixed Vicia faba lateral roots were treated with trypsin, pepsin, RNase, or DNase. Only trypsin affected the morphology of the chromosomes and nuclei. The appearance of the chromosomes after trypsin digestion indicated that each chromatid contained four strands that could be seen with an ordinary light microscope. The experiments are interpreted as indicating that mitotic chromosomes of Vicia faba are multistranded and that the linear continuity of the chromosome is dependent on protein.

Citing Articles

The basis of chromatin fiber assembly within chromosomes studied by histone-DNA crosslinking followed by trypsin digestion.

Goyanes V, Matsui S, Sandberg A Chromosoma. 1980; 78(1):123-35.

PMID: 7379644 DOI: 10.1007/BF00291911.

[Electronmicroscopical studies of human metaphase-chromosomes].

SCHWARZACHER H, Schnedl W Humangenetik. 1967; 4(2):153-65.

PMID: 6078179 DOI: 10.1007/BF00291260.

[Further studies on chromosome proportions and DNA-contents in anurans (Amphibia)].

ULLERICH F Chromosoma. 1967; 21(4):345-68.

PMID: 6056755 DOI: 10.1007/BF00336947.

Electron microscopic observations on interband fibrils in Drosophila salivary chromosomes.

Sorsa M, Sorsa V Chromosoma. 1967; 22(1):32-41.

PMID: 6054310 DOI: 10.1007/BF00291285.

The strandedness of meiotic chromosomes from Oncopeltus.

WOLFE S, Hewitt G J Cell Biol. 1966; 31(1):31-42.

PMID: 5971973 PMC: 2107030. DOI: 10.1083/jcb.31.1.31.

References

Schwartz D . Deoxyribonucleic acid side-chain model of the chromosomes. Nature. 1958; 181(4616):1149-50. DOI: 10.1038/1811149a0. View

Smith H, Fussell C, Kugelman B . Partial Synchronization of Nuclear Divisions in Root Meristems with 5-Aminouracil. Science. 1963; 142(3592):595-6. DOI: 10.1126/science.142.3592.595. View

LA COUR L, RUTISHAUSER A . X-ray breakage experiments with endosperm. I. Sub-chromatid breakage. Chromosoma. 1954; 6(8):696-709. DOI: 10.1007/BF01259956. View

Brooke J, Jenkins D, LAWSON R, Osgood E . Human chromosome uncoiling and dissociation. Ann Hum Genet. 1962; 26:139-43. DOI: 10.1111/j.1469-1809.1962.tb01320.x. View

RIS H . Ultrastructure and molecular organization of genetic systems. Can J Genet Cytol. 1961; 3:95-120. DOI: 10.1139/g61-015. View

Sax K . The effect of ionizing radiation on chromosomes. Q Rev Biol. 1957; 32(1):15-26. DOI: 10.1086/401670. View

Alfert M, Geschwind I . A Selective Staining Method for the Basic Proteins of Cell Nuclei. Proc Natl Acad Sci U S A. 1953; 39(10):991-9. PMC: 1063895. DOI: 10.1073/pnas.39.10.991. View

Peacock W . Chromosome duplication and structure as determined by autoradiography. Proc Natl Acad Sci U S A. 1963; 49:793-801. PMC: 300009. DOI: 10.1073/pnas.49.6.793. View

CAVALIERI L, Rosenberg B . The replication of DNA III. Changes in the number of strands in E. coli DNA during its replication cycle. Biophys J. 1961; 1:337-51. PMC: 1366352. DOI: 10.1016/s0006-3495(61)86893-8. View

10.

SWANSON C . X-Ray and Ultraviolet Studies on Pollen Tube Chromosome: II The Quadripartite Structure of the Prophase Chromosomes of Tradescantia. Proc Natl Acad Sci U S A. 1947; 33(8):229-32. PMC: 1079035. DOI: 10.1073/pnas.33.8.229. View

11.

Steffensen D . A comparative view of the chromosome. Brookhaven Symp Biol. 1959; 12:103-24. View

12.

Meselson M, Stahl F . THE REPLICATION OF DNA IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1958; 44(7):671-82. PMC: 528642. DOI: 10.1073/pnas.44.7.671. View

13.

KAUFMANN B, Gay H, McDonald M . Organizational patterns within chromosomes. Int Rev Cytol. 1960; 9:77-127. DOI: 10.1016/s0074-7696(08)62745-x. View

14.

CROUSE H . X-ray breakage of lily chromosomes at first meiotic metaphase. Science. 1954; 119(3094):485-7. DOI: 10.1126/science.119.3094.485. View

15.

LA COUR L, RUTISHAUSER A . Chromosome breakage experiments with endosperm :sub-chromatid breakage. Nature. 1953; 172(4376):501-2. DOI: 10.1038/172501b0. View

16.

Taylor J, Woods P, HUGHES W . THE ORGANIZATION AND DUPLICATION OF CHROMOSOMES AS REVEALED BY AUTORADIOGRAPHIC STUDIES USING TRITIUM-LABELED THYMIDINEE. Proc Natl Acad Sci U S A. 1957; 43(1):122-8. PMC: 528395. DOI: 10.1073/pnas.43.1.122. View

17.

Steffensen D . Chromosome structure with special reference to the role of metal ions. Int Rev Cytol. 1961; 12:163-97. DOI: 10.1016/s0074-7696(08)60540-9. View

18.

UHL C . CHROMOSOME STRUCTURE AND CROSSING OVER. Genetics. 1965; 51:191-207. PMC: 1210774. DOI: 10.1093/genetics/51.2.191. View

19.

Taylor J . Chromosome reproduction. Int Rev Cytol. 1962; 13:39-73. DOI: 10.1016/s0074-7696(08)60280-6. View