The Replication of DNA III. Changes in the Number of Strands in E. Coli DNA During Its Replication Cycle

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1961 Mar 1

PMID 13691706

Citations 6

Authors

L F CAVALIERI

B H Rosenberg

Affiliations

Soon will be listed here.

Abstract

DNA has been isolated from synchronized cultures of E. coli 15(T)- at various times. At first the DNA was four-stranded (and indistinguishable in all respects from log phase E. coli DNA), but at the start of DNA synthesis the DNA was found to have halved its molecular weight and to have become two-stranded. This sample had all the properties of undenatured, double-helical DNA, and behaved in all respects like DNA from non-proliferating sources. The replication cycle of the DNA molecule has thus been shown to consist of an alternation between the four- and two-stranded forms, the latter being the conserved unit. The evidence provided by the three papers of this series with respect to DNA and chromosomal structure and replication is discussed and summarized.

Citing Articles

The molecular synchrony and sequential replication of DNA in Escherichia coli.

Nagata T Proc Natl Acad Sci U S A. 1963; 49:551-9.

PMID: 13937133 PMC: 299902. DOI: 10.1073/pnas.49.4.551.

Four-stranded DNA as determined by electron microscopy.

Hall C, CAVALIERI L J Biophys Biochem Cytol. 1961; 10:347-51.

PMID: 13710753 PMC: 2225083. DOI: 10.1083/jcb.10.3.347.

The replication of DNA I. Two molecular classes of DNA.

CAVALIERI L, Rosenberg B Biophys J. 1961; 1:317-22.

PMID: 13691704 PMC: 1366350. DOI: 10.1016/s0006-3495(61)86891-4.

[On the time of crossing over and of chromosome duplication in Sphaerocarpus].

Abel W Z Vererbungsl. 1965; 96(3):228-33.

PMID: 5842494

Strandedness of Vicia faba chromosomes as revealed by enzyme digestion studies.

Trosko J, Wolff S J Cell Biol. 1965; 26(1):125-35.

PMID: 5323605 PMC: 2106708. DOI: 10.1083/jcb.26.1.125.

References

MAZIA D . THE PARTICULATE ORGANIZATION OF THE CHROMOSOME. Proc Natl Acad Sci U S A. 1954; 40(6):521-7. PMC: 534082. DOI: 10.1073/pnas.40.6.521. View

Watson J, Crick F . The structure of DNA. Cold Spring Harb Symp Quant Biol. 1953; 18:123-31. DOI: 10.1101/sqb.1953.018.01.020. View

CAVALIERI L, Deutsch J, Rosenberg B . The Molecular Weight and Aggregation of DNA. Biophys J. 2009; 1(4):301-15. PMC: 1366349. DOI: 10.1016/s0006-3495(61)86890-2. View

Bessman M, Lehman I, Simms E, Kornberg A . Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction. J Biol Chem. 1958; 233(1):171-7. View

Watson J, Crick F . Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid. Nature. 1953; 171(4356):737-8. DOI: 10.1038/171737a0. View

Lehman I . Enzymatic synthesis of desoxyribonucleic acid. Ann N Y Acad Sci. 1959; 81:745-56. DOI: 10.1111/j.1749-6632.1959.tb49356.x. View

CAVALIERI L, Rosenberg B . The replication of DNA I. Two molecular classes of DNA. Biophys J. 1961; 1:317-22. PMC: 1366350. DOI: 10.1016/s0006-3495(61)86891-4. View

COHEN S, Arbogast R . Chemical studies in host-virus interactions; a comparison of some properties of three mutant pairs of bacterial viruses, T2r and T2r, T4r and T4r, T6r and T6r. J Exp Med. 1950; 91(6):619-36. PMC: 2136015. DOI: 10.1084/jem.91.6.619. View

Schaechter M, Bentzon M, MAALOE O . Synthesis of deoxyribonucleic acid during the division cycle of bacteria. Nature. 1959; 183(4669):1207-8. DOI: 10.1038/1831207a0. View

10.

DASS C, RIS H . Submicroscopic organization of the nucleus during spermiogenesis in the grasshopper. J Biophys Biochem Cytol. 1958; 4(1):129-32. PMC: 2224325. DOI: 10.1083/jcb.4.1.129. View

11.

Wilkins M, RANDALL J . Crystallinity in sperm heads: molecular structure of nucleoprotein in vivo. Biochim Biophys Acta. 1953; 10(1):192-3. DOI: 10.1016/0006-3002(53)90232-7. View

12.

Taylor J . Asynchronous duplication of chromosomes in cultured cells of Chinese hamster. J Biophys Biochem Cytol. 1960; 7:455-64. PMC: 2224838. DOI: 10.1083/jcb.7.3.455. View

13.

Quastler H . Cell population kinetics. Ann N Y Acad Sci. 1960; 90:580-91. DOI: 10.1111/j.1749-6632.1960.tb23274.x. View

14.

Taylor J . Sister Chromatid Exchanges in Tritium-Labeled Chromosomes. Genetics. 1958; 43(3):515-29. PMC: 1209899. DOI: 10.1093/genetics/43.3.515. View

15.

CAVALIERI L, Rosenberg B . The replication of DNA II. The number of polynucleotide strands in the conserved unit of DNA. Biophys J. 1961; 1:323-36. PMC: 1366351. DOI: 10.1016/s0006-3495(61)86892-6. View

16.

Wilkins M, STOKES A, Wilson H . Molecular structure of deoxypentose nucleic acids. Nature. 1953; 171(4356):738-40. DOI: 10.1038/171738a0. View

17.

Freese E . The arrangement of DNA in the chromosome. Cold Spring Harb Symp Quant Biol. 1958; 23:13-8. DOI: 10.1101/sqb.1958.023.01.005. View

18.

Wilkins M, Battaglia B . Note on the preparation of specimens of oriented sperm heads for x-ray diffraction and infrared absorption studies and on some pseudo-molecular behaviour of sperm. Biochim Biophys Acta. 1953; 11(3):412-5. DOI: 10.1016/0006-3002(53)90060-2. View

19.

Gall J, BJORK L . The spermatid nucleus in two species of grasshopper. J Biophys Biochem Cytol. 1958; 4(4):479-84. PMC: 2224496. DOI: 10.1083/jcb.4.4.479. View

20.

Bessman M, Lehman I, Adler J, Zimmerman S, Simms E, Kornberg A . ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. III. THE INCORPORATION OF PYRIMIDINE AND PURINE ANALOGUES INTO DEOXYRIBONUCLEIC ACID. Proc Natl Acad Sci U S A. 1958; 44(7):633-40. PMC: 528637. DOI: 10.1073/pnas.44.7.633. View