Gene Activation in WI-38 Fibroblasts Stimulated to Proliferate: Requirement for Protein Synthesis

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1971 Aug 1

PMID 5288757

Citations 5

Authors

G Rovera

J Farber

R Baserga

Affiliations

Soon will be listed here.

Abstract

Confluent monolayers of WI-38 human diploid fibroblasts can be stimulated to divide by fresh medium containing 30% fetal-calf serum. Up to 80% of the cells are stimulated to divide, with a peak of DNA synthesis between 15 and 21 hr. 1 hr after the change of medium there is a 70% rise in chromatin template activity. Cycloheximide inhibited the increase in chromatin template activity. A requirement for RNA synthesis was investigated by incubating stimulated and unstimulated cells with 10 mug/ml of actinomycin D. In spite of a 95% inhibition of RNA synthesis in whole cells, purified chromatin from stimulated cells showed the usual increase in template activity. These experiments implicate a requirement for protein synthesis in template activation, and imply that the synthesis of this (or these) protein(s) is independent of RNA synthesis and regulated by a purely translational mechanism.

Citing Articles

Control of cellular proliferation in human diploid fibroblasts.

Baserga R, Rovera G, Farber J In Vitro. 1971; 7(2):80-7.

PMID: 4950443 DOI: 10.1007/BF02628266.

On the recovery of transcription after inhibition by actinomycin D.

Sawicki S, GODMAN G J Cell Biol. 1972; 55(2):299-309.

PMID: 4627744 PMC: 2108806. DOI: 10.1083/jcb.55.2.299.

Changes in the number of binding sites for ribonucleic acid polymerase in chromatin of WI-38 fibroblasts stimulated to proliferate.

Hill B, Baserga R Biochem J. 1974; 141(1):27-34.

PMID: 4616682 PMC: 1168045. DOI: 10.1042/bj1410027.

Changes in chromatin template activity and their relationship to DNA synthesis in mouse parotid glands stimulated by isoproterenol.

NOVI A, Baserga R J Cell Biol. 1972; 55(3):554-62.

PMID: 4571229 PMC: 2108820. DOI: 10.1083/jcb.55.3.554.

Control of transcription of RNA rich in polyadenylic acid in human lymphocytes.

Rosenfeld M, Abrass J, Mendelsohn J, Ross B, Boone R, GARREN L Proc Natl Acad Sci U S A. 1972; 69(8):2306-11.

PMID: 4340753 PMC: 426923. DOI: 10.1073/pnas.69.8.2306.

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