Template Activity of Chromatin During Stimulation of Cellular Proliferation in Human Diploid Fibroblasts

Overview

Journal Biochem J

Specialty Biochemistry

Date 1971 Apr 1

PMID 5165706

Citations 12

Authors

J Farber

G Rovera

R Baserga

Affiliations

Soon will be listed here.

Abstract

1. Contact-inhibited confluent monolayers of WI-38 human diploid fibroblasts can be stimulated to divide by replacing the medium with fresh medium containing 30% foetal calf serum. 2. Of the cells 40-75% are stimulated to divide with a peak DNA synthesis between 15 and 21h and a peak mitotic index between 28 and 30h after stimulation. 3. In the first 12h before the initiation of DNA synthesis there is a biphasic increase in the incorporation of [(3)H]uridine into RNA of whole cells. 4. This is paralleled by a similar biphasic stimulation of chromatin template activity measured in vitro in a system in which purified cell chromatin is incubated with an exogenous RNA polymerase isolated from Escherichia coli. 5. The changes in chromatin template activity are believed to represent activation of the genome, with more sites available for RNA synthesis, and to account almost entirely for the changes in RNA synthesis occurring in the whole cell.

Citing Articles

Gene activation in WI-38 fibroblasts stimulated to proliferate: requirement for protein synthesis.

Rovera G, Farber J, Baserga R Proc Natl Acad Sci U S A. 1971; 68(8):1725-9.

PMID: 5288757 PMC: 389280. DOI: 10.1073/pnas.68.8.1725.

Control of cellular proliferation in human diploid fibroblasts.

Baserga R, Rovera G, Farber J In Vitro. 1971; 7(2):80-7.

PMID: 4950443 DOI: 10.1007/BF02628266.

Changes in the number of binding sites for ribonucleic acid polymerase in chromatin of WI-38 fibroblasts stimulated to proliferate.

Hill B, Baserga R Biochem J. 1974; 141(1):27-34.

PMID: 4616682 PMC: 1168045. DOI: 10.1042/bj1410027.

Increased RNA synthesis in nuclear monolayers of WI-38 cells stimulated to proliferate.

Bombik B, Baserga R Proc Natl Acad Sci U S A. 1974; 71(5):2038-42.

PMID: 4599991 PMC: 388381. DOI: 10.1073/pnas.71.5.2038.

Effect of 5-bromodeoxyuridine on chromatin transcription in confluent fibroblasts.

Hill B, Tsuboi A, Baserga R Proc Natl Acad Sci U S A. 1974; 71(2):455-9.

PMID: 4592691 PMC: 388025. DOI: 10.1073/pnas.71.2.455.

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