A Comparison of Constitutive Heterochromatin Staining Methods in Two Cases of Familial Heterochromatin Deficiencies

Overview

Journal Hum Genet

Specialty Genetics

Date 1979 Nov 1

PMID 527974

Citations 4

Authors

C H Buys

G J ANDERS

W L GOUW

Affiliations

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Abstract

Using DAPI staining after pretreatment with distamycin A we detected a familial deficiency of chromosome 16 heterochromatin. A distinct positively staining band, however, was seen after C-banding. Thus, by using these different heterochromatin staining methods, heterogeneity of the constitutive heterochromatin in the centromeric region of human chromosome 16 was indicated. The same C-banding procedure was also applied to a previously described familial deficiency of chromosome 9 heterochromatin evidenced using distamycin A/DAPI staining and G 11 staining (Buys et al., 1979). In this case a C-band appeared to be virtually absent on the relevant chromosome. These staining methods may be valuable tools in the study of chromosome polymorphisms.

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