Antigen-binding T Cells As Helper Cells. Separation of Helper Cells by Immune Rosette Formation

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1975 Oct 1

PMID 51900

Citations 7

Authors

S Kontiainen

L C Andersson

Affiliations

Soon will be listed here.

Abstract

The spleen T cells from mice immunized 6 days earlier with either chicken gamma globulin (CGG) or with donkey erythrocytes (DRC) were rosetted with CGG-coated sheep erythrocytes or with DRC. The immune rosettes (RFC) (antigen-binding cells) were separated from the bulk of nonrosette-forming cells (non-RFC) by 1-g velocity sedimentation and the RFC and non-RFC tested for helper activity in cooperative antihapten responses in vitro. RFC or non-RFC were mixed with normal or hapten-primed spleen cells, challenged with the appropriate hapten-carrier conjugate and cultured for 4 days in Marbrook tissue cultures. The helping activity was quantitated from the numbers of antihapten antibody-producing cells generated per culture. The results show that specific helper cell activity could be selectively recovered in the immune rosette-forming cell population whereas the non-RFC population was depleted of help. These findings indicate that the helper T cells express specific antigen binding receptors.

Citing Articles

The role of T and B antigen-binding cells in the immune response to sheep erythrocytes in the lizard, Calotes versicolor.

Subramonia Pillai P, Muthukkaruppan V Immunology. 1981; 42(4):541-8.

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Helper and suppressor functions of human mononuclear cells depleted of antigen-binding T8+ cells.

Lehner T, Avery J, Smith R Immunology. 1985; 54(4):701-11.

PMID: 3156809 PMC: 1453565.

Clonal dominance and the preservation of clonal memory cells mediated by antigen-antibody.

Askonas B, McMichael A, Roux M Immunology. 1976; 31(4):541-51.

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T-cell and B-cell identification in the diagnosis of lymphoproliferative disease. A review.

Whiteside T, Rowlands Jr D Am J Pathol. 1977; 88(3):754-92.

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Localization of human T lymphocytes in tissue sections by a rosetting technique.

Brubaker D, Whiteside T Am J Pathol. 1977; 88(2):323-32.

PMID: 327825 PMC: 2032182.

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