Biosynthesis of the Carbohydrate Portions of Immunoglobulin M

Overview

Journal Biochem J

Specialty Biochemistry

Date 1971 Nov 1

PMID 5158908

Citations 15

Authors

R M Parkhouse

F Melchers

Affiliations

Soon will be listed here.

Abstract

Incorporations of radioactive mannose, galactose and fucose into MOPC 104E mouse plasma-cell tumour suspensions suggest a stepwise addition of carbohydrate residues to immunoglobulin M (IgM) during the process of secretion. Mannose and glucosamine residues are added at an early stage, whereas galactose and fucose are added just before, or at the time that, IgM leaves the cell. Free light chains secreted in excess by the same tumour cells incubated with mannose, galactose or fucose contained barely detectable amounts of radioactivity.

Citing Articles

Studies on the secretion of maize root-cap slime : III. Histochemical and autoradiographic localization of incorporated fucose.

Paull R, Jones R Planta. 2014; 127(2):97-110.

PMID: 24430367 DOI: 10.1007/BF00388371.

The confined function model of the Golgi complex: center for ordered processing of biosynthetic products of the rough endoplasmic reticulum.

Tartakoff A Int Rev Cytol. 1983; 85:221-52.

PMID: 6363328 PMC: 7133172. DOI: 10.1016/s0074-7696(08)62374-8.

Lectin-binding sites as markers of Golgi subcompartments: proximal-to-distal maturation of oligosaccharides.

Tartakoff A, Vassalli P J Cell Biol. 1983; 97(4):1243-8.

PMID: 6194163 PMC: 2112602. DOI: 10.1083/jcb.97.4.1243.

Biosynthesis of the carbohydrate portion of immunoglobulins. Incorporation of radioactive fucose into immunoglobulin G1 synthesized and secreted by mouse plasma-cell tumour MOPC 21.

Melchers F Biochem J. 1971; 125(1):241-7.

PMID: 5158909 PMC: 1178046. DOI: 10.1042/bj1250241.

IgM-producing tumors in the BALB-c mouse: a model for B-cell maturation.

Andersson J, Buxbaum J, Citronbaum R, Douglas S, Forni L, Melchers F J Exp Med. 1974; 140(3):742-63.

PMID: 4606641 PMC: 2139610. DOI: 10.1084/jem.140.3.742.

References

Sherr C, UHR J . Immunoglobulin synthesis and secretion. V. Incorporation of leucine and glucosamine into immunoglobulin on free and bound polyribosomes. Proc Natl Acad Sci U S A. 1970; 66(4):1183-9. PMC: 335804. DOI: 10.1073/pnas.66.4.1183. View

Schubert D . Immunoglobulin biosynthesis. IV. Carbohydrate attachment to immunoglobulin subunits. J Mol Biol. 1970; 51(2):287-301. DOI: 10.1016/0022-2836(70)90143-9. View

Jolles P, SAMOUR D, Lederer E . [ISOLATION OF PEPTIDO-GLYCOLIPID FRACTIONS FROM WAX D OF BOVINE, ATYPICAL, AVIAN AND SAPROPHYTIC STRAINS OF MYCOBACTERIA]. Biochim Biophys Acta. 1963; 78:342-50. DOI: 10.1016/0006-3002(63)91644-5. View

Askonas B, Parkhouse R . Assembly of immunoglobulin M. Blocked thiol groups of intracellular 7S subunits. Biochem J. 1971; 123(4):629-34. PMC: 1177004. DOI: 10.1042/bj1230629. View

Parkhouse R . Immunoglobulin M biosynthesis. Production of intermediates and excess of light-chain in mouse myeloma MOPC 104E. Biochem J. 1971; 123(4):635-41. PMC: 1177005. DOI: 10.1042/bj1230635. View

Spragg B, CLAMP J . A study of the carbohydrate present in three type K macroglobulins. Biochem J. 1969; 114(1):57-64. PMC: 1184795. DOI: 10.1042/bj1140057. View

SCHENKEIN I, UHR J . Immunoglobulin synthesis and secretion. I. Biosynthetic studies of the addition of the carbohydrate moieties. J Cell Biol. 1970; 46(1):42-51. PMC: 2108072. DOI: 10.1083/jcb.46.1.42. View

UHR J, SCHENKEIN I . Immunoglobulin synthesis and secretion, IV. Sites of incorporation of sugars as determined by subcellular fractionation. Proc Natl Acad Sci U S A. 1970; 66(3):952-8. PMC: 283143. DOI: 10.1073/pnas.66.3.952. View

WALBORG Jr E, Christensson L, GARDELL S . An ion-exchange column chromatographic method for the separation and quantitative analysis of neutral monosaccharides. Anal Biochem. 1965; 13(2):177-85. DOI: 10.1016/0003-2697(65)90187-9. View

10.

Melchers F . Biosynthesis of the carbohydrate portion of immunoglobulin. Radiochemical and chemical analysis of the carbohydrate moieties of two myeloma proteins purified from different subcellular fractions of plasma cells. Biochemistry. 1971; 10(4):653-9. DOI: 10.1021/bi00780a017. View

11.

Melchers F . Biosynthesis of the carbohydrate portion of immunoglobulins. Kinetics of synthesis and secretion of [3H] leucine-, [3H] galactose- and [3H] mannose-labelled myeloma protein by two plasma-cell tumours. Biochem J. 1970; 119(4):765-72. PMC: 1179464. DOI: 10.1042/bj1190765. View

12.

Melchers F . Biosynthesis of the carbohydrate portion of immunoglobulins. Incorporation of radioactive fucose into immunoglobulin G1 synthesized and secreted by mouse plasma-cell tumour MOPC 21. Biochem J. 1971; 125(1):241-7. PMC: 1178046. DOI: 10.1042/bj1250241. View

13.

Choi Y, Knopf P, Lennox E . Intracellular transport and secretion of an immunoglobulin light chain. Biochemistry. 1971; 10(4):668-79. DOI: 10.1021/bi00780a019. View

14.

McIntimif K, Asofsky R, Potter M, Kuff E . Macroglobulin-Producing Plasma-Cell Tumor in Mice: Identification of a New Light Chain. Science. 1965; 150(3694):361-3. DOI: 10.1126/science.150.3694.361. View

15.

Maizel Jr J, WHITE D, Scharff M . The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology. 1968; 36(1):115-25. DOI: 10.1016/0042-6822(68)90121-9. View

16.

Swenson R, Kern M . THE SYNTHESIS AND SECRETION OF gamma-GLOBULINS BY LYMPH NODE CELLS, I. THE MICROSOMAL COMPARTMENTALIZATION OF gamma-GLOBULINS. Proc Natl Acad Sci U S A. 1967; 57(2):417-22. PMC: 335522. DOI: 10.1073/pnas.57.2.417. View

17.

Eylar E . On the biological role of glycoproteins. J Theor Biol. 1966; 10(1):89-113. DOI: 10.1016/0022-5193(66)90179-2. View

18.

Parkhouse R, Askonas B . Immunoglobulin M biosynthesis. Intracellular accumulation of 7S subunits. Biochem J. 1969; 115(2):163-9. PMC: 1185086. DOI: 10.1042/bj1150163. View