Relationship Between Internal Na + -K + and the Accumulation of 14 C-5-hydroxytryptamine by Rat Platelets

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1971 Dec 1

PMID 5152030

Citations 7

Authors

J M Sneddon

Affiliations

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Abstract

1. 5-Hydroxytryptamine (5-HT) transport has been investigated in rat blood platelets poisoned with dinitrophenol-sodium fluoride or ouabain.2. The inhibition of transport produced by different concentrations of the metabolic inhibitors has been correlated with changes in the internal Na(+) and K(+) concentrations of the platelets.3. Platelets poisoned in a high K(+) medium maintained a high internal K(+) concentration in the absence of cellular metabolism. When transferred to Krebs solutions containing different concentrations of Na(+) they accumulated 5-HT by a process that was related to the magnitudes of the internal and external Na(+) concentrations.4. The results are consistent with the hypothesis that the spontaneous movement of ions through the platelet membrane is capable of providing, at least in part, the energy requirements for 5-HT transport.

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References

White T, Keen P . The role of internal and external Na+ and K+ on the uptake of [3H] noradrenaline by synaptosomes prepared from rat brain. Biochim Biophys Acta. 1970; 196(2):285-95. DOI: 10.1016/0005-2736(70)90016-7. View

Sneddon J . Sodium-dependent accumulation of 5-hydroxytryptamine by rat blood platelets. Br J Pharmacol. 1969; 37(3):680-8. PMC: 1703717. DOI: 10.1111/j.1476-5381.1969.tb08506.x. View

Eddy A, Mulcahy M, Thomson P . The effects of sodium ions and potassium ions on glycine uptake by mouse ascites-tumour cells in the presence and absence of selected metabolic inhibitors. Biochem J. 1967; 103(3):863-76. PMC: 1270492. DOI: 10.1042/bj1030863. View

RIGGS T, Walker L, CHRISTENSEN H . Potassium migration and amino acid transport. J Biol Chem. 1958; 233(6):1479-84. View

KIPNIS D, PARRISH J . Role of Na+ and K+ on sugar (2-deoxyglucose) and amino acid (alpha-aminoisobutyric acid) transport in striated muscle. Fed Proc. 1965; 24(5):1051-9. View

CRANE R . Na+ -dependent transport in the intestine and other animal tissues. Fed Proc. 1965; 24(5):1000-6. View

Eddy A, Hogg M . Further observations on the inhibitory effect of extracellular potassium ions on glycine uptake by mouse ascites-tumour cells. Biochem J. 1969; 114(4):807-14. PMC: 1184968. DOI: 10.1042/bj1140807. View

Schultz S, Curran P . Coupled transport of sodium and organic solutes. Physiol Rev. 1970; 50(4):637-718. DOI: 10.1152/physrev.1970.50.4.637. View

White T, Keen P . Effects of inhibitors of (Na plus, plus, K plus)-dependent adenosine triphosphatase on the uptake of norepinephrne by synaptosomes. Mol Pharmacol. 1971; 7(1):40-5. View

10.

BOGDANSKI D, Tissari A, Brodie B . Mechanism of transport and storage of biogenic amines. 3. Effects of sodium and potassium on kinetics of 5-hydroxytryptamine and norepinephrine transport by rabbit synaptosomes. Biochim Biophys Acta. 1970; 219(1):189-99. DOI: 10.1016/0005-2736(70)90074-x. View

11.

MASON R, Saba S . Platelet ATPase activities. I. Ecto-ATPases of intact platelets and their possible role in aggregation. Am J Pathol. 1969; 55(2):215-23. PMC: 2013489. View

12.

VIDAVER G . SOME TESTS OF THE HYPOTHESIS THAT THE SODIUM-ION GRADIENT FURNISHES THE ENERGY FOR GLYCINE-ACTIVE TRANSPORT BY PIGEON RED CELLS. Biochemistry. 1964; 3:803-8. DOI: 10.1021/bi00894a013. View

13.

Lingjaerde O . Uptake of serotonin in blood platelets: Dependence on sodium and chloride, and inhibition by choline. FEBS Lett. 1969; 3(2):103-106. DOI: 10.1016/0014-5793(69)80108-0. View

14.

Eddy A . The effects of varying the cellular and extracellular concentrations of sodium and potassium ions on the uptake of glycine by mouse ascites-tumour cells in the presence and absence of sodium cyanide. Biochem J. 1968; 108(3):489-98. PMC: 1198835. DOI: 10.1042/bj1080489. View

15.

Humphrey J, TOH C . Absorption of serotonin (5-hydroxytryptamine) and histamine by dog platelets. J Physiol. 1954; 124(2):300-4. PMC: 1366267. DOI: 10.1113/jphysiol.1954.sp005108. View

16.

CHRISTENSEN H, RIGGS T . Concentrative uptake of amino acids by the Ehrlich mouse ascites carcinoma cell. J Biol Chem. 1952; 194(1):57-68. View

17.

Weissbach H, REDFIELD B . Factors affecting the uptake of 5-hydroxytryptamine by human platelets in an inorganic medium. J Biol Chem. 1960; 235:3287-91. View

18.

DETWILER T, Zivkovic R . Control of energy metabolism in platelets. A comparison of aerobic and anaerobic metabolism in washed rat platelets. Biochim Biophys Acta. 1970; 197(2):117-26. DOI: 10.1016/0005-2728(70)90022-8. View

19.

Smith M, Ellory J . Sodium-amino acid interactions in the intestinal epithelium. Philos Trans R Soc Lond B Biol Sci. 1971; 262(842):131-40. DOI: 10.1098/rstb.1971.0084. View

20.

Born G, GILLSON R . Studies on the uptake of 5-hydroxytryptamine by blood platelets. J Physiol. 1959; 146(3):472-91. PMC: 1357045. DOI: 10.1113/jphysiol.1959.sp006206. View