[Influence of Hydroxyurea on DNA Synthesis of Human Bone Marrow in Vivo (author's Transl)]

Overview

Journal Klin Wochenschr

Specialty General Medicine

Date 1979 Nov 2

PMID 513612

Authors

R Hartenstein

E ERTL

K Possinger

H EHRHART

Affiliations

Soon will be listed here.

Abstract

Four patients, who suffered from malignant, disseminated tumors but had a morphologically intact bone marrow, where given a peroral Hydroxyurea (HU) infusion in a concentration of about 5 x 10(-4) mol over a period of 10 h. As indicator of the DNA synthesis the 3H-TdR-incorporation of bone marrow, gained by puncture before, during, and/or after HU application, was measured. While using HU, the DNA synthesis was extensively inhibited. Fourteen to 16 h after discontinuing the HU it increased by the factor 1.5 to 2 of the initial value, i.e., before treatment. This is interpreted as a partial synchronization of bone marrow cells where effects of cell depletion and recruitment are not to be entirely excluded, Toxicity, such as the decrease of peripheral blood cells or nausea, did not occur.

References

Rajewsky M, Hulser D, FABRICIUS E . [Studies on synchronisation in vivo: temporary inhibition of DNA synthesis in normal and malignant mammalian cell systems with hydroxyurea]. Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1971; 76(4):266-92. DOI: 10.1007/BF00304031. View

Young C, HODAS S . HYDROXYUREA: INHIBITORY EFFECT ON DNA METABOLISM. Science. 1964; 146(3648):1172-4. DOI: 10.1126/science.146.3648.1172. View

Sinclair W . Hydroxyurea: differential lethal effects on cultured mammalian cells during the cell cycle. Science. 1965; 150(3704):1729-31. DOI: 10.1126/science.150.3704.1729. View

Hartenstein R, Pfundmair K, Possinger K . [Partial synchronization of tumor cells in vivo with hydroxyurea (author's transl)]. Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1977; 88(3):261-72. DOI: 10.1007/BF00305364. View

Rajewsky M . Synchronisation in vivo: kinetics of a malignant cell system following temporary inhibition of DNA synthesis with hydroxyurea. Exp Cell Res. 1970; 60(2):269-76. DOI: 10.1016/0014-4827(70)90514-8. View

Kim J, Gelbard A, Perez A . Action of hydroxyurea on the nucleic acid metabolism and viability of HeLa cells. Cancer Res. 1967; 27(7):1301-5. View

Sauer H, Pelka R, Wilmanns W . [Pharmakokinetics of hydroxy-urea. Therapy of acute myeoblastic leukemias using synchronization and recruitment effects (author' transl)]. Klin Wochenschr. 1976; 54(5):203-9. DOI: 10.1007/BF01469126. View

Pfeiffer S, Tolmach L . Selecting synchronous populations of mammalian cells. Nature. 1967; 213(5072):139-42. DOI: 10.1038/213139a0. View

FABRICIUS E, Rajewsky F . Determination of hydroxyurea in mammalian tissues and blood. Rev Eur Etud Clin Biol. 1971; 16(7):679-83. View

10.

van Putten L, Keizer H, Mulder J . Synchronization in tumour chemotherapy. Eur J Cancer (1965). 1976; 12(2):79-85. DOI: 10.1016/0014-2964(76)90209-7. View

11.

TUBIANA M, FRINDEL E, Vassort F . Critical survey of experimental data on in vivo synchronization by hydroxyurea. Recent Results Cancer Res. 1975; (52):187-205. DOI: 10.1007/978-3-642-80940-8_22. View

12.

Adams R, Lindsay J . Hydroxyurea reversal of inhibition and use as a cell-synchronizing agent. J Biol Chem. 1967; 242(6):1314-7. View

13.

Volm M, Krieg L, Mattern J, Wayss K . Effect of synchronization on chemotherapy of solid transplanted tumours. Eur J Cancer (1965). 1977; 13(10):1099-108. DOI: 10.1016/0014-2964(77)90007-x. View