The Effects of Indomethacin on Calcium, Sodium, Potassium and Magnesium Fluxes in Various Tissues of the Guinea-pig

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1972 Aug 1

PMID 5085240

Citations 15

Authors

B J Northover

Affiliations

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Abstract

1. Isolated tissues of the guinea-pig were bathed with Krebs solution at 37 degrees C and subjected to 100 ms pulses of electrical stimulation for 30 min at a frequency of 0.1 or 1.0 Hz. The tissues were then dried, ashed, and the ash analysed for calcium, sodium, potassium and magnesium.2. Gastric smooth muscle, cardiac and skeletal muscles and brain all showed a gain of sodium and calcium and a loss of potassium in response to electrical stimulation, but there was no significant change in the magnesium content of any of these tissues.3. Indomethacin (0.5 mM) reduced the calcium content of unstimulated gastric smooth muscle and reduced the gain of calcium and sodium in response to electrical stimulation, but slightly increased the net loss of potassium in response to electrical stimulation.4. Gastric smooth muscle which had gained calcium as a result of electrical stimulation, gradually lost it again when stimulation ceased. Indomethacin (0.5 mM) hastened the net loss of calcium from previously stimulated muscle.5. Indomethacin (0.5 mM) failed to alter the calcium, sodium, potassium and magnesium contents of unstimulated cardiac muscle, skeletal muscle and brain. In these tissues indomethacin (0.5 mM) also failed to prevent the changes in the content of these minerals which occurred in response to electrical stimulation.

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References

AIKAWA J, REARDON J . Effect of sodium salicylate on magnesium metabolism in the rabbit. Proc Soc Exp Biol Med. 1966; 122(3):884-7. DOI: 10.3181/00379727-122-31279. View

CALVERT D, BRODY T . Biochemical alterations of liver function by the halogenated hydrocarbons. I. In vitro and in vivo changes and their modification by ethylenediamine tetraacetate. J Pharmacol Exp Ther. 1958; 124(4):273-81. View

Northover B . Mechanism of the inhibitory action of indomethacin on smooth muscle. Br J Pharmacol. 1971; 41(3):540-51. PMC: 1702866. DOI: 10.1111/j.1476-5381.1971.tb08052.x. View

Feinstein M . INHIBITION OF CAFFEINE RIGOR AND RADIOCALCIUM MOVEMENTS BY LOCAL ANESTHETICS IN FROG SARTORIUS MUSCLE. J Gen Physiol. 1963; 47:151-72. PMC: 2195324. DOI: 10.1085/jgp.47.1.151. View

GALLAGHER C, Gupta D, JUDAH J, Rees K . Biochemical changes in liver in acute thioacetamide intoxication. J Pathol Bacteriol. 1956; 72(1):193-201. DOI: 10.1002/path.1700720125. View

Northover B . The antagonism between anti-inflammatory drugs and substances that constrict veins. J Pathol Bacteriol. 1967; 94(1):206-12. DOI: 10.1002/path.1700940129. View

Feinstein M, Paimre M . Pharmacological action of local anesthetics on excitation-contraction coupling in striated and smooth muscle. Fed Proc. 1969; 28(5):1643-8. View

Somlyo A, Somlyo A . Vascular smooth muscle. II. Pharmacology of normal and hypotensive vessels. Pharmacol Rev. 1970; 22(2):249-353. View

EPSTEIN F, Whittam R . The mode of inhibition by calcium of cell-membrane adenosine-triphosphatase activity. Biochem J. 1966; 99(1):232-8. PMC: 1264980. DOI: 10.1042/bj0990232. View

10.

Somlyo A, Somlyo A . Vascular smooth muscle. I. Normal structure, pathology, biochemistry, and biophysics. Pharmacol Rev. 1968; 20(4):197-272. View

11.

Lehninger A . Water uptake and extrusion by mitochondria in relation to oxidative phosphorylation. Physiol Rev. 1962; 42:467-517. DOI: 10.1152/physrev.1962.42.3.467. View

12.

SLATER E, CLELAND K . The effect of calcium on the respiratory and phosphorylative activities of heart-muscle sarcosomes. Biochem J. 1953; 55(4):566-90. PMC: 1269363. DOI: 10.1042/bj0550566. View

13.

Berczi I, Somogyi A . Biochemical studies on the anticalcinotic effect of sodium salicylate. Can J Physiol Pharmacol. 1969; 47(7):597-601. DOI: 10.1139/y69-105. View

14.

Northover B . The effect of drugs on the constriction of isolated depolarized blood vessels in response to calcium or barium. Br J Pharmacol. 1968; 34(2):417-28. PMC: 1703349. DOI: 10.1111/j.1476-5381.1968.tb07062.x. View

15.

Wrogemann K, BLANCHAER M, Jacobson B . A calcium-associated magnesium-responsive defect of respiration and oxidative phosphorylation by skeletal muscle mitochondria of BIO 14.6 dystrophic hamsters. Life Sci II. 1970; 9(20):1167-73. DOI: 10.1016/0024-3205(70)90035-4. View

16.

Romero P, Whittam R . The control by internal calcium of membrane permeability to sodium and potassium. J Physiol. 1971; 214(3):481-507. PMC: 1331851. DOI: 10.1113/jphysiol.1971.sp009445. View

17.

Rees K, SINHA K, SPECTOR W . The pathogenesis of liver injury in carbon tetrachloride and thioacetamide poisoning. J Pathol Bacteriol. 1961; 81:107-18. View

18.

Reynolds E, THIERS R, Vallee B . Mitochondrial function and metal content in carbon tetrachloride poisoning. J Biol Chem. 1962; 237:3546-51. View

19.

Somogyi A, Berczi I, Selye H . Inhibition by salicylates of various calcifying connective tissue reactions. Arch Int Pharmacodyn Ther. 1969; 177(1):211-23. View

20.

Lehninger A . Mitochondria and calcium ion transport. Biochem J. 1970; 119(2):129-38. PMC: 1179333. DOI: 10.1042/bj1190129. View