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Determination of Nucleic Acid Sequence Homologies and Relative Concentrations by a Dot Hybridization Procedure

Overview
Journal Nucleic Acids Res
Publisher Oxford University Press
Specialty Biochemistry
Date 1979 Nov 24
PMID 503860
Citations 420
Authors
F C Kafatos
C W Jones
A Efstratiadis
Affiliations
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Abstract

A dot hybridization method is presented for rapidly determining the relative concentrations of nucleic acids in a mixture, as well as the extent of sequence homology between related RNA or DNA species.

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References
1.
MARMUR J, Doty P . Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962; 5:109-18. DOI: 10.1016/s0022-2836(62)80066-7. View
2.
Hofstetter H, Schambock A, van den Berg J, Weissmann C . Specific excision of the inserted DNA segment from hybrid plasmids constructed by the poly(dA). poly (dT) method. Biochim Biophys Acta. 1976; 454(3):587-91. DOI: 10.1016/0005-2787(76)90286-0. View
3.
Maniatis T, Kee S, Efstratiadis A, Kafatos F . Amplification and characterization of a beta-globin gene synthesized in vitro. Cell. 1976; 8(2):163-82. DOI: 10.1016/0092-8674(76)90001-5. View
4.
Efstratiadis A, Vournakis J, Donis-Keller H, Chaconas G, Dougall D, Kafatos F . End labeling of enzymatically decapped mRNA. Nucleic Acids Res. 1977; 4(12):4165-74. PMC: 343232. DOI: 10.1093/nar/4.12.4165. View
5.
Regier J, Kafatos F, Goodfliesh R, Hood L . Silkmoth chorion proteins: sequence analysis of the products of a multigene family. Proc Natl Acad Sci U S A. 1978; 75(1):390-4. PMC: 411254. DOI: 10.1073/pnas.75.1.390. View