End Labeling of Enzymatically Decapped MRNA

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1977 Dec 1

PMID 202926

Citations 74

Authors

A Efstratiadis

J N Vournakis

H Donis-Keller

G Chaconas

D K Dougall

F C Kafatos

Affiliations

Soon will be listed here.

Abstract

A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-P-ATP and T4 polynucleotide kinase.

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