Chromosomal Location and Properties of Radiation Sensitivity Mutations in Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1970 Aug 1

PMID 4988238

Citations 44

Authors

J A Hoch

C Anagnostopoulos

Affiliations

Soon will be listed here.

Abstract

Transformation and transduction crosses involving recA1, recB2, and urv-1 mutations have shown that these mutations belong to three distinct unlinked genetic loci. The precise position of these loci on the Bacillus subtilis chromosome map has been determined. The behavior of recB2 strains in transformation studies suggested a dominance of recB2(+) function over recB2 and an early expression of this phenotype during transformation. Strains bearing two ultraviolet sensitivity markers possess a phenotype characteristic of the marker with the most adverse effect on recombination. The possibility that the effects of the two mutations are additive was also considered. Results are also presented which show that a phage-induced enzyme is not responsible for the high transducibility of recA1 strains.

Citing Articles

Genetic and enzymic studies on the recombination process in Bacillus subtilis.

Mazza G, Fortunato A, Ferrari E, Canosi U, Falaschi A, Polsinelli M Mol Gen Genet. 1975; 136(1):9-30.

PMID: 16094963 DOI: 10.1007/BF00275445.

Characterization of Lactococcus lactis UV-sensitive mutants obtained by ISS1 transposition.

Duwat P, Cochu A, Ehrlich S, Gruss A J Bacteriol. 1997; 179(14):4473-9.

PMID: 9226255 PMC: 179281. DOI: 10.1128/jb.179.14.4473-4479.1997.

Cloning, nucleotide sequence, and regulation of the Bacillus subtilis nadB gene and a nifS-like gene, both of which are essential for NAD biosynthesis.

Sun D, Setlow P J Bacteriol. 1993; 175(5):1423-32.

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Postreplication repair of ultraviolet-irradiated transforming deoxyribonucleic acid in Bacillus subtilis.

Hadden C J Bacteriol. 1981; 145(1):434-41.

PMID: 6780518 PMC: 217291. DOI: 10.1128/jb.145.1.434-441.1981.

Capacity for postreplication repair correlated with transducibility in Rec- mutants of Bacillus subtilis.

Dodson L, Hadden C J Bacteriol. 1980; 144(2):608-15.

PMID: 6776091 PMC: 294708. DOI: 10.1128/jb.144.2.608-615.1980.

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