Human Immunity to the Meningococcus. 3. Preparation and Immunochemical Properties of the Group A, Group B, and Group C Meningococcal Polysaccharides

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1969 Jun 1

PMID 4977282

Citations 122

Authors

E C Gotschlich

T Y Liu

M S Artenstein

Affiliations

Soon will be listed here.

Abstract

The group-specific polysaccharides of group A and group C meningococci have been isolated by a new procedure which employs the cationic detergent Cetavlon to precipitate these polysaccharides from the whole culture. The A and C polysaccharide prepared by this method are noteworthy because they are of high molecular weight. The main constituent of the A polysaccharide is N-acetyl, O-acetyl mannosamine phosphate; of the C polysaccharide N-acetyl, O-acetyl neuraminic acid. This purification procedure, when applied to cultures of group B organisms, yields a polysaccharide consisting primarily of N-acetyl neuraminic acid. A passive hemagglutination test developed to measure antibodies to the polysaccharides demonstrated the specificity of these antigens. Using a hemagglutination inhibition test, these antigens were again found to be group-specific, and this test could be used for serogrouping meningococcal isolates.

Citing Articles

A Historical Review of Military Medical Strategies for Fighting Infectious Diseases: From Battlefields to Global Health.

Biselli R, Nisini R, Lista F, Autore A, Lastilla M, De Lorenzo G Biomedicines. 2022; 10(8).

PMID: 36009598 PMC: 9405556. DOI: 10.3390/biomedicines10082050.

Phase I safety and immunogenicity study of a Brazilian serogroup B vaccine.

Martins R, Perisse A, Camacho L, Leal M, Maia M, Homma A Braz J Infect Dis. 2021; 25(6):101652.

PMID: 34793713 PMC: 9392203. DOI: 10.1016/j.bjid.2021.101652.

Development of vaccines at the time of COVID-19.

Almond J, Hacker J, Harwood C, Pizza M, Rappuoli R, Ron E Microlife. 2021; 1(1):uqaa003.

PMID: 34235437 PMC: 7798935. DOI: 10.1093/femsml/uqaa003.

The COVID-19 Vaccine Landscape.

Koch T, Fathi A, Addo M Adv Exp Med Biol. 2021; 1318:549-573.

PMID: 33973199 DOI: 10.1007/978-3-030-63761-3_31.

Complement factor D haplodeficiency is associated with a reduced complement activation speed and diminished bacterial killing.

Langereis J, van der Molen R, de Kat Angelino C, Henriet S, de Jonge M, Joosten I Clin Transl Immunology. 2021; 10(4):e1256.

PMID: 33841879 PMC: 8019133. DOI: 10.1002/cti2.1256.

References

Menzel A, RAKE G . STUDIES ON MENINGOCOCCAL INFECTION : XII. IMMUNOCHEMICAL STUDIES ON MENINGOCOCCUS TYPE II. J Exp Med. 2009; 75(4):437-52. PMC: 2135261. DOI: 10.1084/jem.75.4.437. View

Mokrasch L . Analysis of hexose phosphates and sugar mixtures with the anthrone reagent. J Biol Chem. 1954; 208(1):55-9. View

BLACKLOW R, Warren L . Biosynthesis of sialic acids by Neisseria meningitidis. J Biol Chem. 1962; 237:3520-6. View

Watson R, SCHERP H . The specific hapten of group C (group II alpha) meningococcus. I. Preparation and immunological behavior. J Immunol. 1958; 81(4):331-6. View

Watson R, Marinetti G, SCHERP H . The specific hapten of group C (group II alpha) meningococcus. II. Chemical nature. J Immunol. 1958; 81(4):337-44. View

Frantz I . Growth Requirements of the Meningococcus. J Bacteriol. 1942; 43(6):757-61. PMC: 373642. DOI: 10.1128/jb.43.6.757-761.1942. View

Reinhold V, Dunne F, Wriston J, Schwarz M, Sarda L, Hirs C . The isolation of porcine ribonuclease, a glycoprotein, from pancreatic juice. J Biol Chem. 1968; 243(24):6482-94. View

SLATERUS K . Serological typing of meningococci by means of micro-precipitation. Antonie Van Leeuwenhoek. 1961; 27:305-15. DOI: 10.1007/BF02538460. View

SCHERP H, RAKE G . STUDIES ON MENINGOCOCCUS INFECTION : VIII. THE TYPE I SPECIFIC SUBSTANCE. J Exp Med. 2009; 61(6):753-69. PMC: 2133249. DOI: 10.1084/jem.61.6.753. View

10.

Bing D, Weyand J, Stavitsky A . Hemagglutination with aldehyde-fixed erythrocytes for assay of antigens and antibodies. Proc Soc Exp Biol Med. 1967; 124(4):1166-70. DOI: 10.3181/00379727-124-31953. View

11.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

12.

Mergenhagen S, Martin G, Schiffmann E . Studies on an endotoxin of a group C Neisseria meningitidis. J Immunol. 1963; 90:312-7. View

13.

KABAT E, Kaiser H, Sikorski H . PREPARATION OF THE TYPE-SPECIFIC POLYSACCHARIDE OF THE TYPE I MENINGOCOCCUS AND A STUDY OF ITS EFFECTIVENESS AS AN ANTIGEN IN HUMAN BEINGS. J Exp Med. 2009; 80(4):299-307. PMC: 2135467. DOI: 10.1084/jem.80.4.299. View