Dense Cultures of Neisseria Gonorrhoeae in Liquid Medium

Overview

Journal Appl Microbiol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1967 Mar 1

PMID 4961767

Citations 16

Authors

R Brookes

C G HEDEN

Affiliations

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Abstract

Cultivation of Neisseria gonorrhoeae was effected in a conical glass culture vessel surrounded by a constant-temperature water jacket, and with facilities for stirring, aeration, and pH measurement and control. With the use of an aerated peptone-based medium, containing polypropylene glycol to prevent foam build-up, the yields obtained over the pH range from 5.8 to 7.4 were determined. The greatest yield was obtained at pH 6.4 when the dry weight was 1.5 g/liter. At pH 7.2 to 7.6, lysis was extensive.

Citing Articles

Effect of environment on sensitivity of Neisseria gonorrhoeae to Pseudomonas aeruginosa bacteriocins.

Stein D, Hebeler B, Young F Infect Immun. 1980; 29(2):507-11.

PMID: 6783533 PMC: 551147. DOI: 10.1128/iai.29.2.507-511.1980.

SJ-GC, a modified complete medium for growth of Neisseria gonorrhoeae.

Shockley R, Coffee E, Johnston K J Clin Microbiol. 1980; 12(1):35-8.

PMID: 6775007 PMC: 273515. DOI: 10.1128/jcm.12.1.35-38.1980.

Development of a chemically defined medium for growth of Neisseria gonorrhoeae.

Hunter K, McVEIGH I Antonie Van Leeuwenhoek. 1970; 36(2):305-16.

PMID: 4988226 DOI: 10.1007/BF02069032.

Influence of pH on the growth characteristics of Neisseria gonorrhoeae in continuous culture.

Brookes R, SIKYTA B Appl Microbiol. 1967; 15(2):224-7.

PMID: 4961768 PMC: 546882. DOI: 10.1128/am.15.2.224-227.1967.

Preliminary study of colony type stability of Neisseria gonorrhoeae in liquid culture.

JEPHCOTT A Br J Vener Dis. 1972; 48(5):369-75.

PMID: 4630224 PMC: 1048344. DOI: 10.1136/sti.48.5.369.

References

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Gerhardt P, HEDEN C . Concentrated culture of gonococci in clear liquid medium. Proc Soc Exp Biol Med. 1960; 105:49-51. DOI: 10.3181/00379727-105-26005. View

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