Hybrid Formation Between Bacterial Endotoxins

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1967 Jul 1

PMID 4961269

Citations 9

Authors

J A Rudbach

K C MILNER

E Ribi

Affiliations

Soon will be listed here.

Abstract

When endotoxins extracted from enteric bacteria were mixed in the presence of sodium deoxycholate, and the bile salt was subsequently removed by dialysis or by extraction with ethanol, a new type of endotoxin was formed. The latter material was as biologically active as the original endotoxins and possessed a combination of antigenic determinants that were previously unique to each of the individual endotoxins in the mixture. This hybrid formation between endotoxins was detected by immunodiffusion and radioautography and by quantitative precipitation procedures.

Citing Articles

Endotoxin-altering activity of plasma does not affect antigenicity of native protoplasmic polysaccharide.

Rudbach J, Luoma M Infect Immun. 1974; 10(5):1183-4.

PMID: 16558108 PMC: 423080. DOI: 10.1128/iai.10.5.1183-1184.1974.

Some structural and biological properties of Brucella endotoxin.

Leong D, Diaz R, Milner K, Rudbach J, Wilson J Infect Immun. 1970; 1(2):174-82.

PMID: 16557710 PMC: 415874. DOI: 10.1128/iai.1.2.174-182.1970.

Hybridization by deoxycholate treatment or by biological degradation of bacterial endotoxins extracted from smooth and rough strains.

CHEDID L, Parant M, Parant F, Peroux F, Perez J Infect Immun. 1970; 1(1):15-20.

PMID: 16557688 PMC: 415848. DOI: 10.1128/iai.1.1.15-20.1970.

Pathogenic Yersinia enterocolitica strains increase the outer membrane permeability in response to environmental stimuli by modulating lipopolysaccharide fluidity and lipid A structure.

Bengoechea J, Brandenburg K, Arraiza M, Seydel U, Skurnik M, Moriyon I Infect Immun. 2003; 71(4):2014-21.

PMID: 12654821 PMC: 152087. DOI: 10.1128/IAI.71.4.2014-2021.2003.

Brucella abortus and its closest phylogenetic relative, Ochrobactrum spp., differ in outer membrane permeability and cationic peptide resistance.

Velasco J, Bengoechea J, Brandenburg K, Lindner B, Seydel U, Gonzalez D Infect Immun. 2000; 68(6):3210-8.

PMID: 10816465 PMC: 97564. DOI: 10.1128/IAI.68.6.3210-3218.2000.

References

FREEMAN G . The preparation and properties of a specific polysaccharide from Bact. typhosum Ty(2): With an addendum by J. St L. Philpot, From the Department of Biochemistry, Oxford. Biochem J. 1942; 36(3-4):340-56. PMC: 1265700. DOI: 10.1042/bj0360340. View

Ribi E, Anacker R, Brown R, HASKINS W, Malmgren B, MILNER K . Reaction of endotoxin and surfactants. I. Physical and biological properties of endotoxin treated with sodium deoxycholate. J Bacteriol. 1966; 92(5):1493-509. PMC: 276450. DOI: 10.1128/jb.92.5.1493-1509.1966. View

Olins D, Edelman G . RECONSTITUTION OF 7S MOLECULES FROM L AND H POLYPEPTIDE CHAINS OF ANTIBODIES AND GAMMA-GLOBULINS. J Exp Med. 1964; 119:789-815. PMC: 2137744. DOI: 10.1084/jem.119.5.789. View

Morrison T, WEIBULL C . The occurrence of cell wall constituents in stable Proteus L forms. Acta Pathol Microbiol Scand. 1962; 55:475-82. DOI: 10.1111/j.1699-0463.1962.tb04153.x. View

HYER B, McCarthy B, BOLTON E . A MOLECULAR APPROACH IN THE SYSTEMATICS OF HIGHER ORGANISMS. DNA INTERACTIONS PROVIDE A BASIS FOR DETECTING COMMON POLYNUCLEOTIDE SEQUENCES AMONG DIVERSE ORGANISMS. Science. 1964; 144(3621):959-67. DOI: 10.1126/science.144.3621.959. View

McCarthy B, BOLTON E . An approach to the measurement of genetic relatedness among organisms. Proc Natl Acad Sci U S A. 1963; 50:156-64. PMC: 300669. DOI: 10.1073/pnas.50.1.156. View

ROHOLT O, Onoue K, Pressman D . SPECIFIC COMBINATION OF H AND L CHAINS OF RABBIT GAMMA-GLOBULINS. Proc Natl Acad Sci U S A. 1964; 51:173-8. PMC: 300044. DOI: 10.1073/pnas.51.2.173. View

Ribi E, HASKINS W, MILNER K, Anacker R, RITTER D, Goode G . Physicochemical changes in endotoxin associated with loss of biological potency. J Bacteriol. 1962; 84:803-14. PMC: 277962. DOI: 10.1128/jb.84.4.803-814.1962. View

Rudbach J, Anacker R, HASKINS W, Johnson A, MILNER K, Ribi E . Physical aspects of reversible inactivation of endotoxin. Ann N Y Acad Sci. 1966; 133(2):629-43. DOI: 10.1111/j.1749-6632.1966.tb52394.x. View

10.

Anacker R, Finkelstein R, HASKINS W, Landy M, MILNER K, Ribi E . ORIGIN AND PROPERTIES OF NATURALLY OCCURRING HAPTEN FROM ESCHERICHIA COLI. J Bacteriol. 1964; 88:1705-20. PMC: 277477. DOI: 10.1128/jb.88.6.1705-1720.1964. View

11.

Anacker R, Bickel W, HASKINS W, MILNER K, Ribi E, Rudbach J . Frequency of occurrence of native hapten among enterobacterial species. J Bacteriol. 1966; 91(4):1427-33. PMC: 316059. DOI: 10.1128/jb.91.4.1427-1433.1966. View

12.

Fukushi K, Anacker R, HASKINS W, Landy M, MILNER K, Ribi E . EXTRACTION AND PURIFICATION OF ENDOTOXIN FROM ENTEROBACTERIACEAE: A COMPARISON OF SELECTED METHODS AND SOURCES. J Bacteriol. 1964; 87:391-400. PMC: 277021. DOI: 10.1128/jb.87.2.391-400.1964. View

13.

SCHILDKRAUT C, MARMUR J, Doty P . The formation of hybrid DNA molecules and their use in studies of DNA homologies. J Mol Biol. 1961; 3:595-617. DOI: 10.1016/s0022-2836(61)80024-7. View

14.

MILNER K, Anacker R, Fukushi K, HASKINS W, Landy M, Malmgren B . SYMPOSIUM ON RELATIONSHIP OF STRUCTURE OF MICROORGANISMS TO THEIR IMMUNOLOGICAL PROPERTIES. III. STRUCTURE AND BIOLOGICAL PROPERTIES OF SURFACE ANTIGENS FROM GRAM-NEGATIVE BACTERIA. Bacteriol Rev. 1963; 27:352-68. PMC: 441198. DOI: 10.1128/br.27.4.352-368.1963. View

15.

MILNER K, Finkelstein R . Bioassay of endotoxin: correlation between pyrogenicity for rabbits and lethality for chick embryos. J Infect Dis. 1966; 116(5):529-36. DOI: 10.1093/infdis/116.5.529. View

16.

Rudbach J, Anacker R, HASKINS W, MILNER K, Ribi E . Physical structure of a native protoplasmic polysaccharide from Escherichia coli. J Immunol. 1967; 98(1):1-7. View

17.

OUCHTERLONY O . Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958; 5:1-78. View

18.

Ribi E, HASKINS W, Landy M, MILNER K . Preparation and host-reactive properties of endotoxin with low content of nitrogen and lipid. J Exp Med. 1961; 114:647-63. PMC: 2180377. DOI: 10.1084/jem.114.5.647. View