Heat-labile Antigens of Salmonella Enteritidis. I. Extraction of Antigens

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1966 Sep 1

PMID 4958772

Citations 5

Authors

M Milne

F M Collins

Affiliations

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Abstract

Milne, Margaret (University of Adelaide, Adelaide, Australia), and F. M. Collins. Heat-labile antigens of Salmonella enteritidis. I. Extraction of antigens. J. Bacteriol. 92:543-548. 1966.-Salmonella enteritidis strains of high and low mouse virulence were grown in continuous culture. Cell walls were obtained from both strains by sonic disruption, and the washed walls were extracted at 4 C with sodium dodecyl sulfate or sodium deoxycholate. The extracts were eluted with a saline gradient from a diethylaminoethyl cellulose column, and the separated peaks were tested for precipitin activity against rabbit antisera prepared with heator ethyl alcohol-killed vaccines. The separated antigens reacted less intensely with the antiserum to heat-killed cells than they did to the antisera prepared against alcohol-killed vaccine. Little antigenic difference could be detected between the extracts prepared from the virulent and the avirulent organisms.

Citing Articles

Heat-labile antigens of Salmonella enteritidis. II. Mouse-protection studies.

Collins F, Milne M J Bacteriol. 1966; 92(3):549-57.

PMID: 5922532 PMC: 276288. DOI: 10.1128/jb.92.3.549-557.1966.

Serum-mediated resistance induced with immunogenic preparations of Salmonella typhimurium.

Venneman M, BERRY L Infect Immun. 1971; 4(4):374-80.

PMID: 4949497 PMC: 416316. DOI: 10.1128/iai.4.4.374-380.1971.

Continuous cultivation of microorganisms. A review.

Malek I, Ricica J Folia Microbiol (Praha). 1968; 13(1):46-96.

PMID: 4868263 DOI: 10.1007/BF02866861.

Comparative immunogenicity of heat-killed and living oral Salmonella vaccines.

Collins F, CARTER P Infect Immun. 1972; 6(4):451-8.

PMID: 4564282 PMC: 422558. DOI: 10.1128/iai.6.4.451-458.1972.

Salmonella typhimurium virulence genes necessary to exploit the Itys/s genotype of the mouse.

Benjamin Jr W, Turnbough Jr C, Posey B, Briles D Infect Immun. 1986; 51(3):872-8.

PMID: 3512437 PMC: 260979. DOI: 10.1128/iai.51.3.872-878.1986.

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